Human GPR83 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13837-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

GPR83 cDNA Clone Datasheet

GPR83 cDNA Clone Product Information

Gene Bank Ref.ID:	BC067473
cDNA Size:	1272
Gene Synonym:	GIR, GPR72,GPR83
Source:	Human

GPR83 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens G protein-coupled receptor 83 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

GPR83 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GPR83 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GPR83 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTCCCTC ACCTCTTGCT GCTCTGTCTC CTCCCCTTGG TGCGAGCCAC CGAGCCCCAC
61       GAGGGCCGGG CCGACGAGCA GAGCGCGGAG GCGGCCCTGG CCGTGCCCAA TGCCTCGCAC
121      TTCTTCTCTT GGAACAACTA CACCTTCTCC GACTGGCAGA ACTTTGTGGG CAGGAGGCGC
181      TACGGCGCTG AGTCCCAGAA CCCCACGGTG AAAGCCCTGC TCATTGTGGC TTACTCCTTC
241      ATCATTGTCT TCTCACTCTT TGGCAACGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG
301      CGAATGCACT CGGCCACCAG CCTCTTCATC GTCAACCTGG CAGTTGCCGA CATAATGATC
361      ACGCTGCTCA ACACCCCCTT CACTTTGGTT CGCTTTGTGA ACAGCACATG GATATTTGGG
421      AAGGGCATGT GCCATGTCAG CCGCTTTGCC CAGTACTGCT CACTGCACGT CTCAGCACTG
481      ACACTGACAG CCATTGCGGT GGATCGCCAC CAGGTCATCA TGCACCCCTT GAAACCCCGG
541      ATCTCAATCA CAAAGGGTGT CATCTACATC GCTGTCATCT GGACCATGGC TACGTTCTTT
601      TCACTCCCAC ATGCTATCTG CCAGAAATTA TTTACCTTCA AATACAGTGA GGACATTGTG
661      CGCTCCCTCT GCCTGCCAGA CTTCCCTGAG CCAGCTGACC TCTTCTGGAA GTACCTGGAC
721      TTGGCCACCT TCATCCTGCT CTACATCCTG CCCCTCCTCA TCATCTCTGT GGCCTACGCT
781      CGTGTGGCCA AGAAACTGTG GCTGTGTAAT ATGATTGGCG ATGTGACCAC AGAGCAGTAC
841      TTTGCCCTGC GGCGCAAAAA GAAGAAGACC ATCAAGATGT TGATGCTGGT GGTAGTCCTC
901      TTTGCCCTCT GCTGGTTCCC CCTCAACTGC TACGTCCTCC TCCTGTCCAG CAAGGTCATC
961      CGCACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG CACCTGCTAT
1021     AACCCCTTCA TATACTGCTG GCTGAACGAG AACTTCAGGA TTGAGCTAAA GGCATTACTG
1081     AGCATGTGTC AAAGACCTCC CAAGCCTCAG GAGGACAGGC CACCCTCCCC AGTTCCTTCC
1141     TTCAGGGTGG CCTGGACAGA GAAGAATGAT GGCCAGAGGG CCCCCCTTGC CAATAACCTC
1201     CTGCCCACCT CCCAACTCCA GTCTGGGAAG ACAGACCTGT CATCTGTGGA ACCCATTGTG
1261     ACGATGAGTT AG

Quality Control:cDNA sequencing results is available upon request.