|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Homo sapiens G protein-coupled receptor 114 DNA.|
|pMD18-T Simple Vector|
|Identical with the Gene Bank Ref. ID sequence except for the point mutation 292C/T resulting in the amino acid 98Pro substitution by Ser.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human GPR114 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG10854-M-F|
|Human GPR114 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG10854-M-H|
|Human GPR114 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG10854-M-M|
|Human GPR114 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG10854-M-Y|
|Product name||Product name|
GPR114 belongs to the G-protein coupled receptor 2 family. Members of this family share a common molecular architecture which consists of seven transmembrane domains, three extracellular loops, three intracellular loops, an amino-terminal extracellular domain and an intracellular carboxyl terminus. It is thought that light acts as the activating stimulus of a G-protein-coupled receptor (GPCR). GPCRs are expected to have molecular function (G-protein coupled receptor activity) and to localize in various compartments (endoplasmic reticulum membrane, plasma membrane, integral to membrane). Family B of the GPCRs is a small but structurally and functionally diverse group of proteins that includes receptors for polypeptide hormones, molecules thought to mediate intercellular interactions at the plasma membrane and a group of Drosophila proteins that regulate stress responses and longevity. GPR114 contains 1 GPS domain. GPR114 gene has been proposed to participate in processes (G-protein coupled receptor protein signaling pathway, neuropeptide signaling pathway).