GPR114 Protein

G protein-coupled receptor 114 Protein Datasheet

GPR114 Protein Product Information

• Synonym: PGR27, GPR114
• Protein Construction: A DNA sequence encoding the human GPR114 (Q8IZF4) (Met1-Gly184) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Human
• Expression Host: Human Cells

GPR114 Protein QC Testing

• Purity: >94 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 22
• Molecular Mass: The recombinant human GPR114/Fc is a disulfide-linked homodimer. The reduced monomer comprises 404 amino acids and has a predicted molecular mass of 45.6 kDa. The apparent molecular mass of the protein is approximately 56-63 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GPR114 protein

GPR114 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GPR114 Protein Description

GPR114 contains 1 GPS domain and is a member of the G-protein coupled receptor 2 family. G-protein-coupled receptors (GPCRs) share a common molecular architecture which consists of seven transmembrane domains, three extracellular loops, three intracellular loops, an amino-terminal extracellular domain and an intracellular carboxyl terminus. Light is thought to be the activating stimulus of a GPCR. GPCRs are expected to have molecular function (G-protein coupled receptor activity) and to localize in various compartments (endoplasmic reticulum membrane, plasma membrane, integral to membrane). Family B of the GPCRs is a small but structurally and functionally diverse group of proteins that includes receptors for polypeptide hormones, molecules thought to mediate intercellular interactions at the plasma membrane and a group of Drosophila proteins that regulate stress responses and longevity. GPR114 gene has been proposed to participate in processes (G-protein coupled receptor protein signaling pathway, neuropeptide signaling pathway).

References

1. Ota T. et al., 2004, Nat Genet. 36 (1): 40-5.
2. Bjarnadóttir TK. et al., 2005, Genomics. 84 (1): 23-33.
3. Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.