GPA33 Antibody (Antigen Affinity Purified)

GPA33 Antibody ( Antigen Affinity Purified ) Datasheet

GPA33 Antibody Product Information

• Immunogen: Recombinant Mouse GPA33 protein (Catalog#50559-M08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse GPA33 (rM GPA33; Catalog#50559-M08H; NP_067623.1; Met 1-Ile 235). GPA33 specific IgG was purified by Mouse GPA33 affinity chromatography.

GPA33 Antibody Usage Guide

• Specificity: Mouse GPA33
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse GPA33 in WB. Using a DAB detection system, the detection limit for Mouse GPA33 is approximately 0.5 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse GPA33. The detection limit for Mouse GPA33 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

GPA33 Antibody Background

Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 contains oneIg-like C2-type (immunoglobulin-like) domain and oneIg-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.

References

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2. Ritter G., et al., 1997, Biochem. Biophys. Res. Commun. 236: 682-6.
3. Frey,D. et al., 2008, Cancer Biother Radiopharm. 23 (1): 65-73.
4. Rageul,J. et al., 2009,Int J Cancer. 125 (12): 2802-9.