Anti-A33 Antibody

Cat: 50559-RP02
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Anti-A33 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-A33 Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Mouse
Specificity
Mouse A33
Immunogen
Recombinant Mouse GPA33 protein (Catalog#50559-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse GPA33 (rM GPA33; Catalog#50559-M08H; NP_067623.1; Met 1-Ile 235). GPA33 specific IgG was purified by Mouse GPA33 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-A33 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:1000-1:4000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-A33 Antibody (Rabbit Polyclonal antibody) Images
Mouse GPA33 Immunohistochemistry(IHC) 3279
Immunochemical staining of mouse GPA33 in mouse small intestine with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Mouse GPA33 Western blot (WB) 6777

Anti-mGPA33 rabbit polyclonal antibody at 1:500 dilution.

Lane A: HT-29 Membrane Lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:36 kDa

Anti-A33 Antibody Alternative Names
Anti-2010310L10Rik Antibody;Anti-2210401D16Rik Antibody;Anti-BB116197 Antibody
A33 Background Information

Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I  membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
glycoprotein A33 (transmembrane)
References
  • Heath J.K., et al., 1997, Proc. Natl. Acad. Sci. USA. 94:469-74.
  • Ritter G., et al., 1997, Biochem. Biophys. Res. Commun. 236:682-6.
  • Frey,D. et al., 2008, Cancer Biother Radiopharm  23 (1):65-73.
  • Rageul,J. et al., 2009, Int J Cancer 125 (12):2802-9. 
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-A33 Antibody Rabbit Polyclonal ELISA 50559-RP01
    Anti-A33 Antibody Rabbit Monoclonal FCM 11277-R074
    Anti-A33 Antibody Rabbit Monoclonal ELISA,IHC-P 11277-R071
    Anti-A33 Antibody (PE) Rabbit Monoclonal FCM 11277-R074-P
    Anti-A33 Antibody Rabbit Polyclonal ELISA,IHC-P 11277-RP02
    Anti-A33 Antibody Rabbit Monoclonal ELISA 11277-R022
    Anti-A33 Antibody Rabbit Polyclonal ELISA 11277-RP01
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