GOLM1 / GP73 Protein (His Tag)

Golgi Membrane Protein 1 Protein

GOLM1 / GP73 Protein Product Information

• Synonym: GOLM1,RP11-379P1.3, C9orf155, FLJ22634, FLJ23608, GOLPH2, GP73, PSEC0257, bA379P1.3
• Protein Construction: A DNA sequence encoding the human GOLM1 isoform 1 ( Q8NBJ4-1 ) ( Met 1 - Leu 401 ) was expressed, with a polyhistide tag at the N-terminus
• Source: Human
• Expression Host: E. coli

GOLM1 / GP73 Protein QC Testing

• Purity: > 70 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human GOLM1 consisting of 417 amino acids and has a calculated molecular mass of 47.4 kDa. It migrates as an 60 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50 mM Tris, 0.1% Brig, pH 7.8
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GOLM1 protein

GOLM1 / GP73 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GOLM1 / GP73 Protein Description

Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

References

1. Kladney R.D., et al., 2000, Gene 249:53-65.
2. Kladney R.D., et al., 2002, Virology 301:236-246.
3. Ramachandran P., et al., 2006, J. Proteome Res. 5:1493-1503.
4. Zhou W., et al., 2009, J. Proteome Res. 8:5523-5531.