GOLM1 / GP73 Antibody ( FITC )

GOLM1 / GP73 Antibody Datasheet

GOLM1 / GP73 Antibody Product Information

• Immunogen: Recombinant Human GOLM1 / GP73 protein (Catalog#13066-H08H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 024
• Ig Type: Rabbit IgG
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human GOLM1 / GP73 (rh GOLM1 / GP73; Catalog#13066-H08H; Q8NBJ4-1; Val 40-Leu 401) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

GOLM1 / GP73 Antibody Usage Guide

• Specificity: Human GOLM1 / GP73
• Flow Cytometry:
  

  Flow cytometric analysis of Human GOLM1(GP73) expression in HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Rabbit anti-GOLM1(GP73) (13066-R024-F). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

GOLM1 / GP73 Antibody Background

Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to theGOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

References

1. Kladney R.D., et al., 2000,Gene 249:53-65.
2. Kladney R.D., et al., 2002, Virology 301:236-246.
3. Ramachandran P., et al., 2006, J. Proteome Res. 5:1493-1503.
4. Zhou W.,et al., 2009, J. Proteome Res. 8:5523-5531.