|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
GP73 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate2 µL anti-GP73 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-GP73 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 45 kDa
Observed band size: 70 kDa
Anti-GP73 rabbit polyclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell Lysate
Lane B: 293T Whole Cell Lysate
Lane C: Hela Whole Cell Lysate
Lane D: A549 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:45 kDa
Observed band size:72 kDa
(We are unsure as to the identity of these extra bands.)
Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-GOLPH2 Antibody (FITC)||Rabbit||Monoclonal||FCM||13066-R015-F|
|Anti-GOLPH2 Antibody (PE)||Rabbit||Monoclonal||FCM||13066-R024-P|
|Anti-GOLPH2 Antibody (FITC)||Mouse||Monoclonal||FCM||13066-MM22-F|
|Anti-GOLPH2 Antibody (FITC)||Rabbit||Monoclonal||FCM||13066-R024-F|