Anti-GOLPH2 Antibody

Cat: 13066-RP04
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All GOLPH2 Reagents
CRO Services
Product recommended

All GOLPH2 Reagents
Anti-GOLPH2 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-GOLPH2 Antibody
Validated applications
WB,ELISA,IHC-P,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human GOLPH2
Immunogen
Recombinant Human GOLM1 / GP73 protein (Catalog#13066-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human GOLM1/GP73 (rh GOLM1/GP73; Catalog#13066-H07E; Q8NBJ4-1; Met 1-Leu 401). GOLM1/GP73 specific IgG was purified by Human GOLM1/GP73 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GOLPH2 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:2500-1:10000  
ICC-IF 1:300-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GOLPH2 Antibody (Rabbit Polyclonal antibody) Images
Human GOLM1/GP73 Immunohistochemistry(IHC) 4107
Immunochemical staining of human GP73 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Human GOLM1/GP73 Immunohistochemistry(IHC) 4111
Immunochemical staining of human GP73 in human hepatoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Human GOLM1/GP73 Immunoprecipitation(IP) 8153

GP73 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-GP73 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-GP73 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 45 kDa

Observed band size: 70 kDa

Human GOLM1/GP73 Western blot (WB) 13520

Anti-GP73 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lane D: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:72 kDa

(We are unsure as to the identity of these extra bands.)

Human GOLPH2 Immunofluorescence(IF) 19021
Immunofluorescence staining of GP73 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human GP73 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Anti-GOLPH2 Antibody Alternative Names
Anti-bA379P1.3 Antibody;Anti-C9orf155 Antibody;Anti-GOLPH2 Antibody;Anti-GP73 Antibody;Anti-HEL46 Antibody;Anti-PSEC0257 Antibody
GOLPH2 Background Information

Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

Full Name
golgi membrane protein 1
References
  • Kladney R.D., et al.,(2000), GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249:53-65.
  • Clark H.F., et al., (2003), The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.Genome Res. 13:2265-2270.
  • Puri S., et al.,(2002), Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption.Traffic 3:641-653.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-GOLPH2 Antibody Mouse Monoclonal IHC-P 13066-MM12
    Anti-GOLPH2 Antibody (FITC) Rabbit Monoclonal FCM 13066-R015-F
    Anti-GOLPH2 Antibody Mouse Monoclonal ELISA,IHC-P 13066-MM01
    Anti-GOLPH2 Antibody Mouse Monoclonal ELISA 13066-MM06
    Anti-GOLPH2 Antibody (PE) Rabbit Monoclonal FCM 13066-R024-P
    Anti-GOLPH2 Antibody (FITC) Mouse Monoclonal FCM 13066-MM22-F
    Anti-GOLPH2 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 13066-R015
    Anti-GOLPH2 Antibody Rabbit Monoclonal ELISA,IHC-P 13066-R037
    Anti-GOLPH2 Antibody Rabbit Polyclonal ELISA 13066-RP01
    Anti-GOLPH2 Antibody Rabbit Monoclonal ELISA 13066-R005
    Anti-GOLPH2 Antibody Mouse Monoclonal WB,ELISA,ICC/IF,IF 13066-MM30
    Anti-GOLPH2 Antibody (FITC) Rabbit Monoclonal FCM 13066-R024-F
    Anti-GOLPH2 Antibody Rabbit Monoclonal ELISA,FCM 13066-R024
    Anti-GOLPH2 Antibody Rabbit Polyclonal ELISA,IHC-P 13066-T24
    Anti-GOLPH2 Antibody Rabbit Polyclonal ELISA,IHC-P 13066-T56
    Anti-GOLPH2 Antibody Mouse Monoclonal ELISA 13066-MM15
    info info
    添加购物车成功! 添加购物车失败!