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GOLM1 / GP73 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human GOLM1/GP73 Antibody Product Information
Immunogen:Recombinant Human GOLM1 / GP73 protein (Catalog#13066-H07E)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human GOLM1/GP73 (rh GOLM1/GP73; Catalog#13066-H07E; Q8NBJ4-1; Met 1-Leu 401). GOLM1/GP73 specific IgG was purified by Human GOLM1/GP73 affinity chromatography.
Human GOLM1/GP73 Antibody Usage Guide
Specificity:Human GOLM1 / GP73
Application:WB, ELISA, IHC-P, ICC/IF, IF, IP

WB: 2-5 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GP73. The detection limit for Human GP73 is approximately 0.00975 ng/well.

IHC-P: 0.1-2 μg/mL

ICC/IF: 0.5-1.5 μg/mL

IP: 1-4 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human GOLM1/GP73 Antibody WB Application Image
Human GOLM1/GP73 Antibody IHC Application Image
GOLM1 / GP73 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human GP73 in human hepatoma with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
GOLM1 / GP73 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human GP73 in human liver with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
Human GOLM1/GP73 Antibody IP Application Image
Other GOLM1/GP73 Antibody Products
GOLM1/GP73 Background

Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"