GOLM1 / GP73 Antibody (Antigen Affinity Purified)

GOLM1 / GP73 Antibody ( Antigen Affinity Purified ) Datasheet

GOLM1 / GP73 Antibody Product Information

• Immunogen: Recombinant Human GOLM1 / GP73 protein (Catalog#13066-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human GOLM1/GP73 (rh GOLM1/GP73; Catalog#13066-H08H; Q8NBJ4-1; Val 40-Leu 401). GOLM1/GP73 specific IgG was purified by Human GOLM1/GP73 affinity chromatography.

GOLM1 / GP73 Antibody Usage Guide

• Specificity: Human GOLM1 / GP73
• Cross reactivity: Does not react with
  

  Mouse, Rat

• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of human GOLM1 in human liver with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of hepatocyte and interlobular bile duct. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of human GOLM1 in human endometrium with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of epithelium in uterine gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  	liver	endometrium
  13066-RP02	+	+
  Antigen Blocking	-	-

  positive:+
  negtive: -
  No Data:ND
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GOLM1. The detection limit for Human GOLM1 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

GOLM1 / GP73 Antibody Background

Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to theGOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

References

1. Kladney RD. et al., 2000, Gene. 249: 53-65.
2. Kladney RD. et al., 2002, Virology. 301: 236-46.
3. Ramachandran P. et al., 2006, J Proteome Res. 5: 1493-503.
4. Zhou W. et al., 2009, J Proteome Res. 8: 5523-31.