GOLM1 Antibody

GOLM1 Antibody Datasheet

GOLM1 Antibody Product Information

• Immunogen: Recombinant Human GOLM1 / GP73 protein (Catalog#13066-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 024
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human GOLM1 (rh GOLM1; Catalog#13066-H08H; Q8NBJ4-1; Val 40-Leu 401).

GOLM1 Antibody Usage Guide

• Specificity: Human GOLM1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human GOLM1 in WB. Using a DAB detection system, the detection limit for Human GOLM1 is approximately 1 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GOLM1. The detection limit for Human GOLM1 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

GOLM1 Antibody Background

Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to theGOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

References

1. Kladney R.D., et al., 2000,Gene 249:53-65.
2. Kladney R.D., et al., 2002, Virology 301:236-246.
3. Ramachandran P., et al., 2006, J. Proteome Res. 5:1493-1503.
4. Zhou W.,et al., 2009, J. Proteome Res. 8:5523-5531.