Human GMFB Gene cDNA Clone / ORF Clone

GMFB cDNA Clone Datasheet

GMFB cDNA Clone Product Information

• Gene Bank Ref.ID: BC005359
• cDNA Size: 465
• Gene Synonym: GMF；GMFB
• Source: Human

GMFB cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens glia maturation factor, beta DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GMFB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GMFB cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GMFB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGTGAGT CTTTGGTTGT TTGTGATGTT GCCGAAGATT TAGTGGAAAA GCTGAGAAAG
61       TTTCGTTTTC GCAAAGAAAC GAACAACGCT GCTATTATAA TGAAGATTGA CAAGGATAAA
121      CGCCTGGTGG TACTGGATGA GGAGCTTGAG GGCATTTCAC CAGATGAACT TAAAGATGAA
181      CTACCTGAAC GACAACCTCG CTTCATTGTG TATAGTTATA AATATCAACA TGATGATGGA
241      AGAGTTTCAT ATCCTCTGTG CTTTATTTTC TCCAGTCCTG TTGGATGTAA GCCTGAACAA
301      CAGATGATGT ATGCTGGAAG TAAGAATAAG CTAGTCCAGA CAGCTGAACT AACCAAGGTA
361      TTTGAAATAA GAAATACCGA AGACCTAACT GAAGAATGGT TACGTGAGAA ACTTGGATTT
421      TTCACTAATG TGAACTTCTG TGTTTCTAAA GTATTTATGT ATTAA

 

Quality Control:cDNA sequencing results is available upon request.