|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Homo sapiens glia maturation factor, beta DNA.|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human GMFB Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13244-G-F|
|Human GMFB Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13244-G-H|
|Human GMFB Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13244-G-M|
|Human GMFB Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13244-G-N|
|Human GMFB Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13244-G-Y|
|Product name||Product name|
GMFB is a nerve growth factor which belongs to the actin-binding proteins ADF family, GMF subfamily. GMFB is involved in nervous system development, angiogenesis and immune function. It is especially crucial for the nervous system. GMFB causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. It contains 1 ADF-H domain and is phosphorylated after phorbol ester stimulation. GMFB overexpression in astrocytes results in the increase of BDNF production. GMFB expression is increased by exercise, thus BDNF is important for exercise-induction of BDNF.