GLT25D2 Protein (His Tag)

Glycosyltransferase 25 domain containing 2 Protein Datasheet

GLT25D2 Protein Product Information

• Synonym: RP11-498P10.2, C1orf17, FLJ37771, FLJ37873, KIAA0584
• Protein Construction: A DNA sequence encoding the human GLT25D2 (Q8IYK4) （Met 1-Ser 622）was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

GLT25D2 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human GLT25D2 consists of 632 amino acids and predicts a molecular mass of 73.8 kDa. It migrates as an approximately 68 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM Nacl, 10%gly, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GLT25D2 protein

GLT25D2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GLT25D2 Protein Description

Glycosyl transferase 25 domain 2 (GLT25D2) is a glucosyltransferase enzyme expressed only at low levels in the nervous system. Glycosyltransferases are enzymes that act as a catalyst for the transfer of a monosaccharide unit from an activated nucleotide sugar(also known as the "glycosyl donor") to a glycosyl acceptor molecule, usually an alcohol. Glycosyl transferases transfer glycosyl groups onto their substrate. Localization partially defines their function. Glt25D2 enzyme showed a strong galactosyltransferase activity toward various types of collagen and toward the serum mannose-binding lectin MBL which contains a collagen domain.

References

1. Schegg B. et al., 2009, Mol Cell Biol. 29( 4): 943-52.
2. Sricholpech M. et al., 2011,J Biol Chem. 286 (11): 8846-56.
3. O'Connor DH. et al., 2009, Nature. 461 (7266): 923-9.