Anti-GLO1 Antibody

Cat: 50364-RP02
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Anti-GLO1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-GLO1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse GLO1
Immunogen
Recombinant Mouse GLO1 protein (Catalog#50364-M07E)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse GLO1 (rM GLO1; Catalog#50364-M07E; NP_079650.3; Ala 2-Ile 184). GLO1 specific IgG was purified by mouse GLO1 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GLO1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GLO1 Antibody (Rabbit Polyclonal antibody) Images
Mouse GLO1/Glyoxalase 1 Immunoprecipitation(IP) 8234

Mouse GLO1 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg A549 Whole Cell Lysate

Lane C:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-Mouse GLO1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse GLO1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 24 kDa

Observed band size: 24 kDa

Mouse GLO1/Glyoxalase 1 Western blot (WB) 13669

Anti-GLO1 rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lane C: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:21 kDa

Observed band size:23 kDa

(We are unsure as to the identity of these extra bands.)

Anti-GLO1 Antibody Alternative Names
Anti-0610009E22Rik Antibody;Anti-1110008E19Rik Antibody;Anti-2510049H23Rik Antibody;Anti-AW550643 Antibody;Anti-Glo-1 Antibody;Anti-Glo-1r Antibody;Anti-Glo-1s Antibody;Anti-Glo1-r Antibody;Anti-Glo1-s Antibody;Anti-GLY1 Antibody;Anti-Qglo Antibody
GLO1 Background Information

Lactoylglutathione lyase, also known as Methylglyoxalase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, S-D-lactoylglutathione methylglyoxal lyase and GLO1, is a member of the glyoxalase I family. GLO1 / Glyoxalase I is a ubiquitous cellular defense enzyme involved in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Accumulative evidence suggests an important role of GLO1 expression in protection against methylglyoxal-dependent protein adduction and cellular damage associated with diabetes, cancer, and chronological aging. GLO1 / Glyoxalase I has been implicated in anxiety-like behavior in mice and in multiple psychiatric diseases in humans. GLO1 / Glyoxalase I catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. GLO1 / Glyoxalase I exists in three separable isoforms which originate from two alleles in the genome. These correspond to two homodimers and one heterodimer composed of two subunits showing different electrophoretic properties. GLO1 upregulation may play a functional role in glycolytic adaptations of cancer cells.

Full Name
glyoxalase I
References
  • Wu, YY. et al., 2008,Prog Neuropsychopharmacol Biol Psychiatry. 32 (7):1740-4.
  • Williams,R. et al., 2009, PLoS One  4 (3): e4649.
  • Antognelli,C. et al., 2009, BMC Cancer  9 :115.
  • Bair,W.B. et al., 2010, Melanoma Res  20 (2):85-96.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-GLO1 Antibody Rabbit Polyclonal ELISA 50364-RP01
    Anti-GLO1 Antibody Rabbit Monoclonal ELISA 50364-R043
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