Human GLIS1 Gene cDNA Clone / ORF Clone

Human GLIS1 cDNA Clone Datasheet

GLIS1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_147193.2
• cDNA Size: 1863
• Gene Synonym: FLJ36155
• Source: Human

GLIS1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens GLIS family zinc finger 1 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1483 C/T resulting in the amino acid pro substitution by leu and 966 C/T not causing the amino acid variation.

GLIS1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GLIS1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GLIS1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGAGG CCCGCACATC CCTGTCTGCC CACTGTCGGG GCCCGCTGGC CACTGGCCTG
61       CACCCAGACC TGGACCTCCC GGGCCGAAGC CTCGCCACCC CTGCGCCTTC CTGCTACCTT
121      CTGGGCAGCG AACCCAGCTC TGGCCTGGGC CTCCAGCCCG AGACCCACCT CCCCGAGGGC
181      AGCCTGAAGC GGTGCTGCGT CTTGGGCCTA CCCCCCACCT CCCCAGCCTC CTCCTCACCC
241      TGTGCCTCCT CCGACGTCAC CTCCATCATC CGCTCCTCCC AGACGTCTCT GGTCACCTGT
301      GTAAATGGAC TCCGGAGCCC CCCTCTGACG GGAGATCTGG GGGGCCCTTC CAAGCGGGCC
361      CGGCCTGGCC CTGCATCGAC GGACAGCCAT GAGGGCAGCT TGCAACTTGA AGCCTGCCGG
421      AAGGCGAGCT TCCTGAAGCA GGAACCCGCG GATGAGTTTT CAGAGCTCTT TGGGCCTCAC
481      CAGCAGGGCC TGCCGCCCCC CTATCCCCTG TCTCAGTTGC CGCCTGGCCC AAGCCTTGGA
541      GGCCTGGGGC TGGGCCTGGC AGGCAGGGTG GTGGCCGGGC GGCAGGCGTG CCGCTGGGTG
601      GACTGCTGTG CAGCCTATGA GCAGCAGGAG GAGCTGGTGC GGCACATCGA GAAGAGCCAC
661      ATCGACCAGC GCAAGGGCGA GGACTTCACC TGCTTCTGGG CTGGCTGCGT GCGCCGCTAC
721      AAGCCCTTCA ACGCCCGCTA CAAGCTGCTC ATCCACATGC GAGTGCACTC GGGCGAGAAG
781      CCCAACAAGT GCATGTTTGA AGGCTGCAGC AAGGCCTTCT CACGGCTGGA GAACCTCAAG
841      ATCCACCTGA GGAGCCACAC GGGCGAGAAG CCGTACCTGT GCCAGCACCC GGGTTGCCAG
901      AAGGCCTTCA GCAACTCCAG CGACCGCGCC AAGCACCAGC GCACCCACCT AGACACGAAG
961      CCGTACGCCT GTCAGATCCC TGGCTGCTCC AAGCGCTACA CAGACCCCAG CTCCCTCCGC
1021     AAGCACGTCA AGGCCCATTC AGCCAAAGAG CAGCAGGTGC GTAAGAAGCT GCATGCGGGC
1081     CCTGACACCG AGGCCGACGT CCTGACCGAG TGTCTGGTCC TGCAGCAGCT CCACACGTCC
1141     ACACAGCTGG CTGCCAGCGA CGGCAAGGGT GGCTGTGGCC TGGGCCAGGA GCTGCTCCCA
1201     GGTGTGTATC CTGGCTCCAT CACCCCCCAT AACGGACTTG CATCGGGCCT CCTGCCCCCA
1261     GCGCACGACG TACCTTCCAG GCACCACCCG CTGGATGCCA CCACCAGTTC CCACCACCAT
1321     CTGTCCCCTC TGCCCATGGC TGAGAGCACC CGGGATGGGT TGGGGCCCGG CCTCCTCTCA
1381     CCAATAGTCA GCCCCCTGAA GGGGCTGGGG CCACCGCCGC TGCCCCCATC CTCTCAGAGC
1441     CATTCTCCGG GGGGCCAGCC CTTCCCCACA CTCCCCAGCA AGCCGTCCTA CCCACCCTTC
1501     CAGAGCCCTC CACCCCCGCC TCTGCCCAGC CCACAAGGTT ACCAGGGCAG TTTCCACTCC
1561     ATCCAGAGTT GCTTCCCCTA TGGCGACTGC TACCGGATGG CTGAACCAGC AGCCGGTGGG
1621     GACGGACTGG TCGGGGAGAC CCACGGTTTC AACCCCCTGC GGCCCAATGG CTACCACAGC
1681     CTCAGCACGC CCTTGCCTGC CACAGGCTAT GAGGCCCTGG CTGAGGCCTC ATGCCCCACA
1741     GCGCTGCCAC AGCAGCCATC TGAAGATGTG GTGTCCAGCG GCCCCGAGGA CTGTGGCTTC
1801     TTCCCCAATG GAGCCTTTGA CCACTGCCTG GGCCACATCC CCTCCATCTA CACAGACACC
1861     TGA

 

Quality Control:cDNA sequencing results is available upon request.