GLIPR1 / RTVP1 Protein (His Tag)

Glioma Pathogenesis-Related Protein 1

GLIPR1 / RTVP1 Protein Product Information

• Synonym: CRISP7, GLIPR, RTVP1, GLIPR1
• Protein Construction: A DNA sequence encoding the human GLIPR1 (NP_006842.2) extracellular domain ( Met 1-Arg 232 ) was expressed, with a C-terminal polyhistidine tag
• Source: Human
• Expression Host: Human Cells

GLIPR1 / RTVP1 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 22
• Molecular Mass: The secreted recombinant human GLIPR1 comprises 222 amino acids and has a predicted molecular mass of 25.7 kDa. rhGLIPR1 migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
• SDS-PAGE: GLIPR1 protein

GLIPR1 / RTVP1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GLIPR1 / RTVP1 Protein Description

Glioma pathogenesis-related protein 1, also known as Protein RTVP-1, GLIPR1 and GLIPR, is a single-pass membrane protein which belongs to the CRISP family. GLIPR1 / RTVP-1 was expressed in high levels in glioblastomas, whereas its expression in low-grade astrocytomas and normal brains was very low. Transfection of glioma cells with small interfering RNAs targeting GLIPR1 / RTVP-1 decreased cell proliferation in all the cell lines examined and induced cell apoptosis in some of them. Overexpression of GLIPR1 / RTVP-1 increased astrocyte and glioma cell proliferation and the anchorage-independent growth of the cells. In addition, overexpression of GLIPR1 / RTVP-1 rendered glioma cells more resistant to the apoptotic effect of tumor necrosis factor-related apoptosis-inducing ligand and serum deprivation. GLIPR1 / RTVP-1 regulated the invasion of glioma cells was evident by their enhanced migration through Matrigel and by their increased invasion in a spheroid confrontation assay. The increased invasive potential of the GLIPR1 / RTVP-1 overexpressors was also shown by the increased activity of matrix metalloproteinase 2 in these cells. The expression of GLIPR1 / RTVP-1 is correlated with the degree of malignancy of astrocytic tumors and that GLIPR1 / RTVP-1 is involved in the regulation of the growth, survival, and invasion of glioma cells. GLIPR1 / RTVP-1 is a potential therapeutic target in gliomas.

References

1. Murphy E.V., et al., 1995, Gene. 159:131-5.
2. Rich T., et al., 1996, Gene. 180:125-30.
3. Rosenzweig,T. et al., 2006, Cancer Res. 66 (8):4139-48.