Human GLB1 Gene cDNA Clone / ORF Clone

GLB1 cDNA Clone Datasheet

GLB1 cDNA Clone Product Information

• Gene Bank Ref.ID: BC007493
• cDNA Size: 2034
• Gene Synonym: EBP;ELNR1;MPS4B;GLB1
• Source: Human

GLB1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens galactosidase, beta 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GLB1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GLB1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GLB1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCGGGGT TCCTGGTTCG CATCCTCCTT CTGCTGCTGG TTCTGCTGCT TCTGGGCCCT
61       ACGCGCGGCT TGCGCAATGC CACCCAGAGG ATGTTTGAAA TTGACTATAG CCGGGACTCC
121      TTCCTCAAGG ATGGCCAGCC ATTTCGCTAC ATCTCAGGAA GCATTCACTA CTCCCGTGTG
181      CCCCGCTTCT ACTGGAAGGA CCGGCTGCTG AAGATGAAGA TGGCTGGGCT GAACGCCATC
241      CAGACGTATG TGCCCTGGAA CTTTCATGAG CCCTGGCCAG GACAGTACCA GTTTTCTGAG
301      GACCATGATG TGGAATATTT TCTTCGGCTG GCTCATGAGC TGGGACTGCT GGTTATCCTG
361      AGGCCCGGGC CCTACATCTG TGCAGAGTGG GAAATGGGAG GATTACCTGC TTGGCTGCTA
421      GAGAAAGAGT CTATTCTTCT CCGCTCCTCC GACCCAGATT ACCTGGCAGC TGTGGACAAG
481      TGGTTGGGAG TCCTTCTGCC CAAGATGAAG CCTCTCCTCT ATCAGAATGG AGGGCCAGTT
541      ATAACAGTGC AGGTTGAAAA TGAATATGGC AGCTACTTTG CCTGTGATTT TGACTACCTG
601      CGCTTCCTGC AGAAGCGCTT TCGCCACCAT CTGGGGGATG ATGTGGTTCT GTTTACCACT
661      GATGGAGCAC ATAAAACATT CCTGAAATGT GGGGCCCTGC AGGGCCTCTA CACCACGGTG
721      GACTTTGGAA CAGGCAGCAA CATCACAGAT GCTTTCCTAA GCCAGAGGAA GTGTGAGCCC
781      AAAGGACCCT TGATCAATTC TGAATTCTAT ACTGGCTGGC TAGATCACTG GGGCCAACCT
841      CACTCCACAA TCAAGACCGA AGCAGTGGCT TCCTCCCTCT ATGATATACT TGCCCGTGGG
901      GCGAGTGTGA ACTTGTACAT GTTTATAGGT GGGACCAATT TTGCCTATTG GAATGGGGCC
961      AACTCACCCT ATGCAGCACA GCCCACCAGC TACGACTATG ATGCCCCACT GAGTGAGGCT
1021     GGGGACCTCA CTGAGAAGTA TTTTGCTCTG CGAAACATCA TCCAGAAGTT TGAAAAAGTA
1081     CCAGAAGGTC CTATCCCTCC ATCTACACCA AAGTTTGCAT ATGGAAAGGT CACTTTGGAA
1141     AAGTTAAAGA CAGTGGGAGC AGCTCTGGAC ATTCTGTGTC CCTCTGGGCC CATCAAAAGC
1201     CTTTATCCCT TGACATTTAT CCAGGTGAAA CAGCATTATG GGTTTGTGCT GTACCGGACA
1261     ACACTTCCTC AAGATTGCAG CAACCCAGCA CCTCTCTCTT CACCCCTCAA TGGAGTCCAC
1321     GATCGAGCAT ATGTTGCTGT GGATGGGATC CCCCAGGGAG TCCTTGAGCG AAACAATGTG
1381     ATCACTCTGA ACATAACAGG GAAAGCTGGA GCCACTCTGG ACCTTCTGGT AGAGAACATG
1441     GGACGTGTGA ACTATGGTGC ATATATCAAC GATTTTAAGG GTTTGGTTTC TAACCTGACT
1501     CTCAGTTCCA ATATCCTCAC GGACTGGACG ATCTTTCCAC TGGACACTGA GGATGCAGTG
1561     CGCAGCCACC TGGGGGGCTG GGGACACCGT GACAGTGGCC ACCATGATGA AGCCTGGGCC
1621     CACAACTCAT CCAACTACAC GCTCCCGGCC TTTTATATGG GGAACTTCTC CATTCCCAGT
1681     GGGATCCCAG ACTTGCCCCA GGACACCTTT ATCCAGTTTC CTGGATGGAC CAAGGGCCAG
1741     GTCTGGATTA ATGGCTTTAA CCTTGGCCGC TATTGGCCAG CCCGGGGCCC TCAGTTGACC
1801     TTGTTTGTGC CCCAGCACAT CCTGATGACC TCGGCCCCAA ACACCATCAC CGTGCTGGAA
1861     CTGGAGTGGG CACCCTGCAG CAGTGATGAT CCAGAACTAT GTGCTGTGAC GTTCGTGGAC
1921     AGGCCAGTTA TTGGCTCATC TGTGACCTAC GATCATCCCT CCAAACCTGT TGAAAAAAGA
1981     CTCATGCCCC CACCCCCGCA AAAAAACAAA GATTCATGGC TGGACCATGT ATGA

 

Quality Control:cDNA sequencing results is available upon request.