Rat GITR / TNFRSF18 Protein (Cytokine)

Tumor necrosis factor receptor superfamily, member 18 Protein Datasheet

GITR / TNFRSF18 Protein Product Information

• Synonym: TNFRSF18
• Protein Construction: A DNA sequence encoding the rat TNFRSF18 (Q5M835) (Met1-Lys121) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

GITR / TNFRSF18 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 25
• Molecular Mass: The recombinant rat TNFRSF18/Fc is a disulfide-linked homodimer. The reduced monomer comprises 338 amino acids and has a predicted molecular mass of 37.7 kDa. The apparent molecular mass of the protein is approximately 47 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GITR / TNFRSF18 protein

GITR / TNFRSF18 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GITR / TNFRSF18 Protein Description

GITR, also known as TNFRSF18(CD357), belongs to the tumor necrosis factor receptor (TNF-R) superfamily. It is the receptor for TNFSF18. GITR and its ligand are important costimulatory molecules in the pathogenesis of autoimmune diseases. It also mediates NF-kappa-B activation via the TRAF2/NIK pathway. GITR plays a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. GITR may be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death.

References

1. Won B. et al., 1999, J Biol Chem. 274 (10): 6056-61.
2. Nocentini G. et al., 1997, Proc Natl Acad Sci. 94 (12): 6216-21.
3. Kim YS. et al., 2007, Oncol Rep. 18 (5): 1189-94.