Human GIF Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13544-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

GIF cDNA Clone Datasheet

GIF cDNA Clone Product Information

Gene Bank Ref.ID:	BC037958
cDNA Size:	1254
Gene Synonym:	IF;INF;IFMH;TCN3;GIF
Source:	Human

GIF cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens gastric intrinsic factor (vitamin B synthesis) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

GIF cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GIF cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GIF cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCTGGT TTGCCCTCTA CCTCCTGAGC CTTCTCTGGG CTACAGCTGG GACTAGTACC
61       CAGACCCAGA GTTCATGCTC CGTTCCCTCA GCACAGGAGC CCTTGGTCAA TGGAATACAA
121      GTACTCATGG AGAACTCGGT GACTTCATCA GCCTACCCAA ACCCCAGCAT CCTGATTGCC
181      ATGAATCTGG CCGGAGCCTA CAACTTGAAG GCCCAGAAGC TCCTGACTTA CCAGCTCATG
241      TCCAGCGACA ACAACGATCT AACCATTGGG CAGCTCGGCC TCACCATCAT GGCCCTCACC
301      TCCTCCTGCC GAGACCCTGG GGATAAAGTA TCCATTCTAC AAAGACAAAT GGAGAACTGG
361      GCACCTTCCA GCCCCAACGC TGAAGCATCA GCCTTCTATG GGCCCAGTCT AGCGATCTTG
421      GCACTGTGCC AGAAGAACTC TGAGGCGACC TTGCCGATAG CCGTCCGCTT TGCCAAGACC
481      CTGCTGGCCA ACTCCTCTCC CTTCAATGTA GACACAGGAG CAATGGCAAC CTTGGCTCTG
541      ACCTGTATGT ACAACAAGAT CCCTGTAGGT TCAGAGGAAG GTTACAGATC CCTGTTTGGT
601      CAGGTACTAA AGGATATTGT GGAGAAAATC AGCATGAAGA TCAAAGATAA TGGCATCATT
661      GGAGACATCT ACAGTACTGG CCTCGCCATG CAGGCTCTCT CTGTAACACC TGAGCCATCT
721      AAAAAGGAAT GGAACTGCAA GAAGACTACG GATATGATAC TCAATGAGAT TAAGCAGGGG
781      AAATTCCACA ACCCCATGTC CATTGCTCAA ATCCTCCCTT CCCTGAAAGG CAAGACATAC
841      CTAGATGTGC CCCAGGTCAC TTGTAGTCCT GATCATGAGG TACAACCAAC TCTACCCAGC
901      AACCCTGGCC CTGGCCCCAC CTCTGCATCT AACATCACTG TCATATACAC CATAAATAAC
961      CAGCTGAGGG GGGTTGAGCT GCTCTTCAAC GAGACCATCA ATGTTAGTGT GAAAAGTGGG
1021     TCAGTGTTAC TTGTTGTCCT AGAGGAAGCA CAGCGCAAAA ATCCTATGTT CAAATTTGAA
1081     ACCACAATGA CATCTTGGGG CCTTGTCGTC TCTTCTATCA ACAATATCGC GGAAAATGTT
1141     AATCACAAGA CATACTGGCA GTTTCTTAGT GGTGTAACAC CTTTGAATGA AGGGGTTGCT
1201     GACTACATAC CCTTCAACCA CGAGCACATC ACAGCCAATT TCACACAGTA CTAA

Quality Control:cDNA sequencing results is available upon request.