Human GHRL Gene cDNA Clone / ORF Clone

GHRL cDNA Clone Datasheet

GHRL cDNA Clone Product Information

• Gene Bank Ref.ID: BC025791
• cDNA Size: 354
• Gene Synonym: MTLRP; GHRL
• Source: Human

GHRL cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens ghrelin/obestatin prepropeptide DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GHRL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GHRL cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GHRL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG
61       GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG
121      TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA
181      GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAAATGGAAG TCCGGTTCAA CGCCCCCTTT
241      GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG
301      TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

 

Quality Control:cDNA sequencing results is available upon request.