Mouse GFRA3 Gene cDNA Clone / ORF Clone

GFRA3 cDNA Clone Datasheet

GFRA3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010280.3
• cDNA Size: 1194
• Gene Synonym: Y15110; Gfra3
• Source: Mouse

GFRA3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus glial cell line derived neurotrophic factor family receptor alpha 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GFRA3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GFRA3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GFRA3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGCTCT CCTGGAGCCC GCGACCTCCA CTGCTGATGA TCCTGCTACT GGTGCTGTCG
61       TTGTGGCTGC CACTTGGAGC AGGAAACTCC CTTGCCACAG AGAACAGGTT TGTGAACAGC
121      TGTACCCAGG CCAGAAAGAA ATGCGAGGCT AATCCCGCTT GCAAGGCTGC CTACCAGCAC
181      CTGGGCTCCT GCACCTCCAG TTTAAGCAGG CCGCTGCCCT TAGAGGAGTC TGCCATGTCT
241      GCAGACTGCC TAGAGGCAGC AGAACAACTC AGGAACAGCT CTCTGATAGA CTGCAGGTGC
301      CATCGGCGCA TGAAGCACCA AGCTACCTGT CTGGACATTT ATTGGACCGT TCACCCTGCC
361      CGAAGCCTTG GTGACTACGA GTTGGATGTC TCACCCTATG AAGACACAGT GACCAGCAAA
421      CCCTGGAAAA TGAATCTTAG CAAGTTGAAC ATGCTCAAAC CAGACTCGGA CCTCTGCCTC
481      AAATTTGCTA TGCTGTGTAC TCTTCACGAC AAGTGTGACC GCCTGCGCAA GGCCTACGGG
541      GAGGCATGCT CAGGGATCCG CTGCCAGCGC CACCTCTGCC TAGCCCAGCT GCGCTCCTTC
601      TTTGAGAAGG CAGCAGAGTC CCACGCTCAG GGTCTGCTGC TGTGTCCCTG TGCACCAGAA
661      GATGCGGGCT GTGGGGAGCG GCGGCGTAAC ACCATCGCCC CCAGTTGCGC CCTGCCTTCT
721      GTAACCCCCA ATTGCCTGGA TCTGCGGAGC TTCTGCCGTG CGGACCCTTT GTGCAGATCA
781      CGCCTGATGG ACTTCCAGAC CCACTGTCAT CCTATGGACA TCCTTGGGAC TTGTGCAACT
841      GAGCAGTCCA GATGTCTGCG GGCATACCTG GGGCTGATTG GGACTGCCAT GACCCCAAAC
901      TTCATCAGCA AGGTCAACAC TACTGTTGCC TTAAGCTGCA CCTGCCGAGG CAGCGGCAAC
961      CTACAGGACG AGTGTGAACA GCTGGAAAGG TCCTTCTCCC AGAACCCCTG CCTCGTGGAG
1021     GCCATTGCAG CTAAGATGCG TTTCCACAGA CAGCTCTTCT CCCAGGACTG GGCAGACTCT
1081     ACTTTTTCAG TGGTGCAGCA GCAGAACAGC AACCCTGCTC TGAGACTGCA GCCCAGGCTA
1141     CCCATTCTTT CTTTCTCCAT CCTTCCCTTG ATTCTGCTGC AGACCCTCTG GTAG

 

Quality Control:cDNA sequencing results is available upon request.