GFRA3 / GFR-alpha-3 Protein (His Tag)

GDNF Family Receptor alpha-3 ( GFRA3 / GFRα3 ) Protein

GFRA3 / GFRα3 Protein Product Information

• Synonym: GFRA3
• Protein Construction: A DNA sequence encoding the human GFRa3 ( NP_001487.2 ) ( Met 1 - Trp 382 ) was expressed, fused with the a C-terminal polyhistidine tag
• Source: Human
• Expression Host: Human Cells

GFRA3 / GFRα3 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 32
• Molecular Mass: The recombinant human GFRa3 consists of 362 amino acids and predicts a molecular mass of 40.8 kDa. As a result of glycosylation, the apparent molecular mass of rhGFRa3 is approximately 50 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GFRA3 protein

GFRA3 / GFRα3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GFRA3 / GFRα3 Protein Description

GDNF family receptor alpha-3, also known as GFR-alpha 3, and GFRA3, is GPI-linked cell surface receptor which belongs to the GDNFR family. GFR-alpha 3 forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. GFR-alpha 3 (GFRa3) is widely expressed in adult and fetus which exhibit a similar pattern. It is essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. GFR-alpha 3 (GFRa3) is moderately expressed in many non-neuronal tissues, particularly those of the digestive and urogenital systems. The GDNF family receptor alpha (GFR-alpha) proteins are extracellular cell surface bound molecules that act as adapters in binding of the GDNF family of soluble neurotrophic factors to the RET receptor. These molecules are essential for development of many neural crest derived cell types and the kidney. Members of the GFR-alpha family are also candidates for Hirschsprung disease (HSCR) mutations.

References

1. Jing, S. et al., 1998,  J Biol Chem. 272 (52): 33111-7.
2. Nomoto, S. et al.,1998, Biochem Biophys Res Commun.244(3):849-53.
3. Onochie, CI. et al., 2000, J. Med. Genet. 37 (9): 674-9.
4. Gimm, O. et al., 2001, Oncogene 20 (17): 2161-70.