GFRA3 / GFR-alpha-3 Antibody (Antigen Affinity Purified)

GDNF Family Receptor Alpha-3 Antibody ( Antigen Affinity Purified )

GFRA3 / GFR-alpha-3 Antibody Product Information

• Immunogen: Recombinant human GFRa3 protein ( Catalog#10213-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human GFRA3 ( rh GFRa3 ; Catalog#10213-H08H ; NP_001487.2 ; Met 1 - Trp 382 ). GFRa3 specific IgG was purified by human GFRa3 affinity chromatography

GFRA3 / GFR-alpha-3 Antibody Usage Guide

• Specificity: Human GFRA3 / GFR-alpha-3
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human GFRa3 in WB. Using a DAB detection system, the detection limit for human GFRa3 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human GFRa3. The detection limit for human GFRa3 is approximately 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

GFRA3 / GFR-alpha-3 Antibody Background

GDNF family receptor alpha-3, also known as GFR-alpha 3, and GFRA3, is GPI-linked cell surface receptor which belongs to the GDNFR family. GFR-alpha 3 forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. GFR-alpha 3 (GFRa3) is widely expressed in adult and fetus which exhibit a similar pattern. It is essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. GFR-alpha 3 (GFRa3) is moderately expressed in many non-neuronal tissues, particularly those of the digestive and urogenital systems. The GDNF family receptor alpha (GFR-alpha) proteins are extracellular cell surface bound molecules that act as adapters in binding of the GDNF family of soluble neurotrophic factors to the RET receptor. These molecules are essential for development of many neural crest derived cell types and the kidney. Members of the GFR-alpha family are also candidates for Hirschsprung disease (HSCR) mutations

References

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2. Nomoto, S. et al.,1998, Biochem Biophys Res Commun.244(3):849-53.
3. Onochie, CI. et al., 2000, J. Med. Genet. 37 (9): 674-9.
4. Gimm, O. et al., 2001, Oncogene 20 (17): 2161-70.