Mouse GFRA2 Gene cDNA Clone / ORF Clone

GFRA2 cDNA Clone Datasheet

GFRA2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008115.2
• cDNA Size: 1395
• Gene Synonym: Gfra2
• Source: Mouse

GFRA2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus glial cell line derived neurotrophic factor family receptor alpha 2 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for four point mutations: 195 A/C, 576 C/T, 738 A/G and 750 T/C not causing the amino acid variation.

GFRA2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GFRA2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

GFRA2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATCTTGG CAAACGCCTT CTGCCTCTTC TTCTTTTTAG ACGAAACCCT CCGCTCTTTG
61       GCCAGCCCTT CCTCTCCGCA GGGCTCTGAG CTCCACGGCT GGCGCCCCCA AGTGGACTGT
121      GTCCGGGCCA ATGAGCTGTG TGCGGCTGAA TCCAACTGCA GCTCCAGGTA CCGCACCCTT
181      CGGCAGTGCC TGGCCGGCCG GGATCGCAAT ACCATGCTGG CCAATAAGGA GTGCCAGGCG
241      GCCCTGGAGG TCTTGCAGGA AAGCCCATTG TATGACTGCC GCTGCAAGCG GGGCATGAAG
301      AAGGAGCTGC AGTGTCTGCA GATCTATTGG AGCATCCATC TGGGGCTGAC GGAGGGTGAG
361      GAGTTCTACG AAGCTTCGCC CTATGAGCCT GTGACCTCCC GCCTCTCGGA CATCTTCAGG
421      CTCGCTTCAA TCTTCTCAGG GACAGGGGCA GACCCGGTGG TCAGTGCCAA GAGCAACCAC
481      TGCCTGGATG CCGCCAAGGC CTGCAACCTG AACGACAACT GCAAGAAGCT CCGCTCCTCC
541      TACATCTCCA TCTGCAACCG CGAGATCTCT CCCACTGAAC GCTGCAACCG CCGCAAGTGC
601      CACAAGGCCC TGCGCCAGTT CTTCGACCGT GTGCCCAGCG AGTATACCTA CCGCATGCTC
661      TTCTGCTCCT GTCAGGACCA GGCATGCGCC GAGCGTCGCC GGCAAACCAT CCTGCCCAGC
721      TGTTCCTATG AGGACAAGGA GAAGCCCAAC TGCTTGGACC TGCGCAGCCT GTGTCGTACA
781      GACCACTTGT GCCGGTCCCG CCTGGCAGAC TTCCACGCCA ACTGTCGAGC CTCCTACCGG
841      ACAATCACCA GCTGCCCTGC GGACAACTAC CAGGCATGTC TGGGCTCCTA TGCTGGCATG
901      ATTGGGTTTG ATATGACACC GAACTATGTG GACTCCAACC CCACGGGCAT CGTGGTGTCT
961      CCCTGGTGCA ATTGTCGTGG CAGTGGGAAC ATGGAAGAAG AGTGTGAGAA GTTCCTCAAG
1021     GACTTCACAG AAAACCCATG CCTCCGGAAT GCCATTCAAG CCTTTGGCAA TGGCACAGAT
1081     GTGAACATGT CTCCCAAAGG CCCCACATTT TCAGCTACCC AGGCCCCTCG GGTAGAGAAA
1141     ACTCCTTCAC TGCCAGATGA CCTCAGTGAT AGCACCAGCC TGGGGACCAG TGTCATCACC
1201     ACCTGCACAT CTATCCAGGA GCAAGGGCTG AAGGCCAACA ACTCCAAAGA GTTAAGCATG
1261     TGTTTCACAG AGCTCACGAC AAATATCAGC CCAGGGAGTA AAAAGGTGAT CAAACTTTAC
1321     TCAGGCTCCT GCAGAGCCAG ACTGTCGACT GCCTTGACTG CCCTCCCACT CCTGATGGTG
1381     ACCTTGGCCC AGTAG

 

Quality Control:cDNA sequencing results is available upon request.