Rat GFRA1 Gene cDNA Clone / ORF Clone

GFRA1 cDNA Clone Datasheet

GFRA1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_012959.1
• cDNA Size: 1407
• Gene Synonym: Gfra1
• Source: Rat

GFRA1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus GDNF family receptor alpha 1 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

GFRA1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GFRA1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GFRA1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTCCTAG CCACTCTGTA CTTCGCGCTG CCACTCCTGG ATTTGCTGAT GTCCGCCGAG
61       GTGAGTGGTG GAGACCGTCT GGACTGTGTG AAAGCCAGCG ATCAGTGCCT GAAGGAACAG
121      AGCTGCAGCA CCAAGTACCG CACACTAAGG CAGTGCGTGG CGGGCAAGGA AACCAACTTC
181      AGCCTGACAT CCGGCCTTGA GGCCAAGGAT GAGTGCCGTA GCGCCATGGA GGCCTTGAAG
241      CAGAAGTCTC TGTACAACTG CCGCTGCAAG CGGGGCATGA AGAAAGAGAA GAATTGTCTG
301      CGTATCTACT GGAGCATGTA CCAGAGCCTG CAGGGAAATG ACCTCCTGGA AGATTCCCCG
361      TATGAGCCGG TTAACAGCAG GTTGTCAGAT ATATTCCGGG CAGTCCCGTT CATATCAGAT
421      GTTTTCCAGC AAGTGGAACA CATTTCCAAA GGGAACAACT GCCTGGACGC AGCCAAGGCC
481      TGCAACCTGG ACGACACCTG TAAGAAGTAC AGGTCGGCCT ACATCACCCC CTGCACCACC
541      AGCATGTCCA ACGAGGTCTG CAACCGCCGT AAGTGCCACA AGGCCCTCAG GCAGTTCTTC
601      GACAAGGTTC CGGCCAAGCA CAGCTACGGG ATGCTCTTCT GCTCCTGCCG GGACATCGCC
661      TGCACCGAGC GGCGGCGACA GACTATCGTC CCCGTGTGCT CCTATGAAGA ACGAGAGAGG
721      CCCAACTGCC TGAGTCTGCA AGACTCCTGC AAGACCAATT ACATCTGCAG ATCTCGCCTT
781      GCAGATTTTT TTACCAACTG CCAGCCAGAG TCAAGGTCTG TCAGCAACTG TCTTAAGGAG
841      AACTACGCAG ACTGCCTCCT GGCCTACTCG GGACTGATTG GCACAGTCAT GACTCCCAAC
901      TACGTAGACT CCAGCAGCCT CAGCGTGGCA CCATGGTGTG ACTGCAGCAA CAGCGGCAAT
961      GACCTGGAAG ACTGCTTGAA ATTTCTGAAT TTTTTTAAGG ACAATACTTG TCTCAAAAAT
1021     GCAATTCAAG CCTTTGGCAA TGGCTCAGAT GTGACCATGT GGCAGCCAGC CCCTCCAGTC
1081     CAGACCACCA CTGCCACCAC TACCACTGCC TTCCGGGTCA AGAACAAGCC TCTGGGGCCA
1141     GCAGGGTCTG AGAATGAGAT CCCCACACAC GTTTTACCAC CCTGTGCGAA TTTGCAGGCT
1201     CAGAAGCTGA AATCCAATGT GTCGGGTAGC ACACACCTCT GTCTTTCTGA TAGTGATTTC
1261     GGAAAGGATG GTCTCGCTGG TGCCTCCAGC CACATAACCA CAAAATCAAT GGCTGCTCCT
1321     CCCAGCTGCA GTCTGAGCTC ACTGCCGGTG CTGATGCTCA CCGCCCTTGC TGCCCTGTTA
1381     TCTGTATCGT TGGCAGAAAC GTCGTAG

 

Quality Control:cDNA sequencing results is available upon request.