Mouse GFRA1 / GFR alpha-1 Protein (His Tag)

Glial Cell line Derived Neurotrophic Factor Family Receptor Alpha 1

GFRA1 Protein Product Information

• GFRA1 Synonym: Gfra1,AU042498
• Protein Construction: A DNA sequence encoding the extracellular domain ( Met 1 - Ser 425 ) of mouse GFRα1 isoform beta ( AAB86600.1 ) was expressed with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

GFRA1 Protein QC Testing

• Purity: > 98%, as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 25
• Molecular Mass: The secreted recombinant mouse GFRα1consists of 412 amino acids and has a calculated molecular mass of 46.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GFRA1 protein

GFRA1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GFRA1 Protein Description

Mouse GDNF family receptor alpha 1 ( GFRα-1) is a glycosyl phosphatidylinositol (GPI)-linked protein that binds Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGFβ superfamily of growth factors. Three receptors for these factors, GFRα-1, GFRα-2 and GFRα-3 have been identified. The receptors do not contain transmembrane domains and are attached to the cell membrane by GPI linkage. Both GFRα-1 and GFRα-2 have been shown to mediate the GDNF-dependent and NTN-dependent phosphorylation and activation of the tyrosine kinase Ret which is necessary for kidney and enteric neuron development. GFRα-1 is the primary receptor for GDNF and GFRα-2 exhibits a preference for NTN. GFRα-3 is expressed only during development. GFRα-1 gene is a candidate gene for Hirschsprung disease.

References

1. Lin, L. F. et al., 1993. Science. 260: 1130-1132.
2. Jing, S. et al., 1996. Cell. 85: 1113-1124.
3. Treanor, J. J. et al., 1996. Nature. 382: 80-83.
4. Naveilhan, P. et al., 1998. Proc. Natl. Acad. Sci. USA. 95: 1295-1300.
5. Dey, B.K. et al., 1998, NeuroReport. 9: 37-42.