Rat GFRA1 / GFR alpha-1 Protein (His Tag)

Rat GFRA1 / GFR alpha-1 Protein

Rat GFRA1 / GFR alpha-1 Protein Product Information

• Synonym: Gfra1
• Protein Construction: A DNA sequence encoding the rat GFRA1 ( Q62997-1 ) ( Met 1 - Leu 445 ) was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Rat
• Expression Host: Human Cells

Rat GFRA1 / GFR alpha-1 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 25
• Molecular Mass: The recombinant rat GFRA1 comprises 432 amino acids and predicts a molecular mass of 48 kDa. The apparent molecular mass of the rat GFRA1 is approximately 58-65 kDa in SDS-PAGE under reducing conditions due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GFRA1 protein

Rat GFRA1 / GFR alpha-1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Rat GFRA1 / GFR alpha-1 Protein Description

Rat GFR alpha -1 is a novel glycosylphosphatidylinositol (GPI)-linked cell surface receptor, and belongs to the glial cell line-derived neurotrophic factor (GDNF) receptor subfamily consisting of at least four members termed GFR alpha 1-4. The GDNF family ligands comprises GDNF, Neurturin (NTN), Artemin, and Persephin, and upon the specifically GDNF binding with high affinity, GFRα-1 mediates the association with and activation of the RET tyrosine kinase receptor (RTK), and subsequently initiates the RET signaling pathway. GFR alpha-mediated signaling plays an important role in the survival, differentiation, and migration of central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system, as well as the inflammatory response in some carciniomas. In addition, recent studies have revealed that GFRα-1 also associates with the neural cell adhesion molecule NCAM and promotes high affinity GDNF binding to NCAM, and accordingly down-regulates NCAM-mediated homophilic cell adhesion via the RET-independent signaling.

References

1. Jing, S. et al., 1996, Cell. 85: 1113-24.
2. Treanor, J.J. et al., 1996, Nature. 382: 80-3.
3. Paratcha, G. et al., 2003, Cell. 113: 867-79.
4. Esseqhir, S. et al., 2007, Cancer. Res. 67: 11732-41.
5. Sjostrand, D. et al., 2008, J. Biol. Chem. 283: 13792-8.
6. Lee, R.H. et al., 2006, J. Neurosci. Res. 83: 80-90.