GFRA1 Antibody

GFRA1 Antibody Datasheet

GFRA1 Antibody Product Information

• Immunogen: Recombinant Mouse GFRA1 protein (Catalog#50171-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 013
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse GFRA1 (rM GFRA1; Catalog#50171-M08H; AAB86600.1; Met 1-Ser 425).

GFRA1 Antibody Usage Guide

• Specificity: Mouse GFRA1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse GFRa1 in WB. Using a DAB detection system, the detection limit for Mouse GFRa1 is approximately 5 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

GFRA1 Antibody Background

GFR alpha -1 is a novel glycosylphosphatidylinositol (GPI)-linked cell surface receptor, and belongs to the glial cell line-derived neurotrophic factor (GDNF) receptor subfamily consisting of at least four members termed GFR alpha 1-4. The GDNF family ligands comprises GDNF, Neurturin (NTN), Artemin, and Persephin, and upon the specifically GDNF binding with high affinity, GFRα-1 mediates the association with and activation of the RET tyrosine kinase receptor (RTK), and subsequently initiates the RET signaling pathway. GFR alpha-mediated signaling plays an important role in the survival, differentiation, and migration of central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system, as well as the inflammatory response in some carciniomas. In addition, recent studies have revealed that GFRα-1 also associates with the neural cell adhesion molecule NCAM and promotes high affinity GDNF binding to NCAM, and accordingly down-regulates NCAM-mediated homophilic cell adhesion via the RET-independent signaling.

References

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3. Treanor, J. J. et al., 1996. Nature. 382: 80-83.
4. Naveilhan, P. et al., 1998. Proc. Natl. Acad. Sci. USA. 95: 1295-1300.
5. Dey, B.K. et al., 1998, NeuroReport. 9: 37-42.