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GFP Antibody, Mouse MAb

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Aequorea victoria GFP Antibody Product Information
Immunogen:Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
Clone ID:05
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser 2-Lys 238). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Aequorea victoria GFP Antibody Usage Guide
Specificity:Aequorea victoria GFP
No cross-reactivity in ELISA with
E.coli cell lysate
Application:ELISA, IP, ICC/IF, IF

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Aequorea victoria GFP. The detection limit for Aequorea victoria GFP is approximately 0.16 ng/well.

ICC/IF: 10-25 μg/mL

IP: 1-4 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Aequorea victoria GFP Antibody IF Application Image
GFP Antibody, Mouse Mab, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of GFP protein in CHO cells, transfected with GFP. Cells (left: GFP, middle: antibody, right: merge) were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-GFP monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue).
Aequorea victoria GFP Antibody IP Application Image
Other GFP Antibody Products
Reactivity: Aequorea victoria  
Application: ELISA  WB  IP  
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13105-R208-50
13105-R208-100
50 µL 
100 µL 
Add to Cart
Reactivity: Aequorea victoria  
Application: ELISA  
    13105-T16-100
    13105-T16-50
    13105-T16-200
    100 µg 
    50 µg 
    200 µg 
    Add to Cart
    Reactivity: Aequorea victoria  
    Application: ELISA  
      13105-RP01-100
      13105-RP01-400
      13105-RP01-200
      100 µg 
       
      200 µg 
      Add to Cart
      Green fluorescent protein / GFP Background

      The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

      Aequorea victoria Green fluorescent protein / GFP References
    • Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637.
    • Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248.
    • Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067.
    • Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028.
    • Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161.
    • Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.
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      Size / Price
      Catalog: 13105-MM05-50
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      Size:
      50 µg
      100 µg
      Quantity:+-
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      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"