Anti-GFP Tag Antibody

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Anti-GFP Tag Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-GFP Tag Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Aequorea victoria
Specificity
Aequorea victoria GFP Tag
Immunogen
Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser2-Lys238).
Source
Monoclonal Rabbit IgG Clone #208
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GFP Tag Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
WB 1/1000-1/5000
ELISA 1/10000-1/20000
IP 1-4 μl/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GFP Tag Antibody (Rabbit Monoclonal antibody) Images

Anti-GFP Tag was immunoprecipitated using:

Lane A: GFP transfected E.coli cell lysate (0.5mg)

Lane B: GFP transfected 293 cell lysate (0.5mg)

 

Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilution

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GFP rabbit monoclonal antibody at 1:500 dilution

Lane A: GFP transfected E.coli lysate (5ug)

Lane B: GFP transfected 293 Cell lysate (30ug)

Lane C: Non-transfected 293 Cells lysate (30ug)

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:27 kDa

Observed band size:27 kDa

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