Human GFI1 Gene cDNA Clone / ORF Clone

Human GFI1 cDNA Clone Datasheet

GFI1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_005263.3
• cDNA Size: 1269
• Gene Synonym: SCN2; GFI-1; ZNF163; FLJ94509; GFI1
• Source: Human

GFI1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens growth factor independent 1 transcription repressor DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 465 C/T not causing the amino acid variation.

GFI1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GFI1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GFI1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCGCGCT CATTTCTCGT CAAAAGCAAG AAGGCTCACA GCTACCACCA GCCGCGCTCC
61       CCAGGACCAG ACTATTCCCT CCGTTTAGAG AATGTACCGG CGCCTAGCCG AGCAGACAGC
121      ACTTCAAATG CAGGCGGGGC GAAGGCGGAG CCCCGGGACC GTTTGTCCCC CGAATCGCAG
181      CTGACCGAAG CCCCAGACAG AGCCTCCGCA TCCCCAGACA GCTGCGAAGG CAGCGTCTGC
241      GAACGGAGCT CGGAGTTTGA GGACTTCTGG AGGCCCCCGT CACCCTCCGC GTCTCCAGCC
301      TCGGAGAAGT CAATGTGCCC ATCGCTGGAC GAAGCCCAGC CCTTCCCCCT GCCTTTCAAA
361      CCGTACTCAT GGAGCGGCCT GGCGGGTTCT GACCTGCGGC ACCTGGTGCA GAGCTACCGA
421      CCGTGTGGGG CCCTGGAGCG TGGCGCTGGC CTGGGCCTCT TCTGTGAACC CGCCCCGGAG
481      CCTGGCCACC CGGCCGCGCT GTACGGCCCG AAGCGGGCTG CCGGCGGCGC GGGGGCCGGG
541      GCGCCAGGGA GCTGCAGCGC AGGGGCCGGT GCCACCGCTG GCCCTGGCCT AGGGCTCTAC
601      GGCGACTTCG GGTCTGCGGC AGCCGGGCTG TATGAGAGGC CCACGGCAGC GGCGGGCTTG
661      CTGTACCCCG AGCGTGGCCA CGGGCTGCAC GCAGACAAGG GCGCTGGCGT CAAGGTGGAG
721      TCGGAGCTGC TGTGCACCCG CCTGCTGCTG GGCGGCGGCT CCTACAAGTG CATCAAGTGC
781      AGCAAGGTGT TCTCCACGCC GCACGGGCTC GAGGTGCACG TGCGCAGGTC CCACAGCGGT
841      ACCAGACCCT TTGCCTGCGA GATGTGCGGC AAGACCTTCG GGCACGCGGT GAGCCTGGAG
901      CAGCACAAAG CCGTGCACTC GCAGGAACGG AGCTTTGACT GTAAGATCTG TGGGAAGAGC
961      TTCAAGAGGT CATCCACACT GTCCACACAC CTGCTTATCC ACTCAGACAC TCGGCCCTAC
1021     CCCTGTCAGT ACTGTGGCAA GAGGTTCCAC CAGAAGTCAG ACATGAAGAA ACACACTTTC
1081     ATCCACACTG GTGAGAAGCC TCACAAGTGC CAGGTGTGCG GCAAGGCATT CAGCCAGAGC
1141     TCCAACCTCA TCACCCACAG CCGCAAACAC ACAGGCTTCA AGCCCTTCGG CTGCGACCTC
1201     TGTGGGAAGG GTTTCCAGAG GAAGGTGGAC CTCCGAAGGC ACCGGGAGAC GCAGCATGGG
1261     CTCAAATGA

 

Quality Control:cDNA sequencing results is available upon request.