Human GFAP Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12167-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

GFAP cDNA Clone Datasheet

GFAP cDNA Clone Product Information

Gene Bank Ref.ID:	BC013596
cDNA Size:	1299
Gene Synonym:	FLJ42474; FLJ45472;GFAP
Source:	Human

GFAP cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens glial fibrillary acidic protein DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

GFAP cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GFAP cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GFAP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGAGGA GACGCATCAC CTCCGCTGCT CGCCGCTCCT ACGTCTCCTC AGGGGAGATG
61       ATGGTGGGGG GCCTGGCTCC TGGCCGCCGT CTGGGTCCTG GCACCCGCCT CTCCCTGGCT
121      CGAATGCCCC CTCCACTCCC GACCCGGGTG GATTTCTCCC TGGCTGGGGC ACTCAATGCT
181      GGCTTCAAGG AGACCCGGGC CAGTGAGCGG GCAGAGATGA TGGAGCTCAA TGACCGCTTT
241      GCCAGCTACA TCGAGAAGGT TCGCTTCCTG GAACAGCAAA ACAAGGCGCT GGCTGCTGAG
301      CTGAACCAGC TGCGGGCCAA GGAGCCCACC AAGCTGGCAG ACGTCTACCA GGCTGAGCTG
361      CGAGAGCTGC GGCTGCGGCT CGATCAACTC ACCGCCAACA GCGCCCGGCT GGAGGTTGAG
421      AGGGACAATC TGGCACAGGA CCTGGCCACT GTGAGGCAGA AGCTCCAGGA TGAAACCAAC
481      CTGAGGCTGG AAGCCGAGAA CAACCTGGCT GCCTATAGAC AGGAAGCAGA TGAAGCCACC
541      CTGGCCCGTC TGGATCTGGA GAGGAAGATT GAGTCGCTGG AGGAGGAGAT CCGGTTCTTG
601      AGGAAGATCC ACGAGGAGGA GGTTCGGGAA CTCCAGGAGC AGCTGGCCCG ACAGCAGGTC
661      CATGTGGAGC TTGACGTGGC CAAGCCAGAC CTCACCGCAG CCCTGAAAGA GATCCGCACG
721      CAGTATGAGG CAATGGCGTC CAGCAACATG CATGAAGCCG AAGAGTGGTA CCGCTCCAAG
781      TTTGCAGACC TGACAGACGC TGCTGCCCGC AACGCGGAGC TGCTCCGCCA GGCCAAGCAC
841      GAAGCCAACG ACTACCGGCG CCAGTTGCAG TCCTTGACCT GCGACCTGGA GTCTCTGCGC
901      GGCACGAACG AGTCCCTGGA GAGGCAGATG CGCGAGCAGG AGGAGCGGCA CGTGCGGGAG
961      GCGGCCAGTT ATCAGGAGGC GCTGGCGCGG CTGGAGGAAG AGGGGCAGAG CCTCAAGGAC
1021     GAGATGGCCC GCCACTTGCA GGAGTACCAG GACCTGCTCA ATGTCAAGCT GGCCCTGGAC
1081     ATCGAGATCG CCACCTACAG GAAGCTGCTA GAGGGCGAGG AGAACCGGAT CACCATTCCC
1141     GTGCAGACCT TCTCCAACCT GCAGATTCGA GAAACCAGCC TGGACACCAA GTCTGTGTCA
1201     GAAGGCCACC TCAAGAGGAA CATCGTGGTG AAGACCGTGG AGATGCGGGA TGGAGAGGTC
1261     ATTAAGGAGT CCAAGCAGGA GCACAAGGAT GTGATGTGA

Quality Control:cDNA sequencing results is available upon request.