Product name
Anti-GFAP Antibody
Validated applications
IHC-P
Species reactivity
Reacts with: Human
Predicted to React with:
Rat (Species predicted to react based on 100% sequence homology)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human GFAP.
Preparation
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the Human GFAP, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Immunochemical staining of human GFAP in human glioma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human GFAP in human clear cell tumors (brain) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunochemical staining of human GFAP in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
GFAP is a cell-specific marker which belongs to the intermediate filament family. It can distinguish astrocytes from other glial cells during development. GFAP is expressed in cells lacking fibronectin. It is a type III intermediate filaments protein which contains three domains: the head, rod and tail domains. GFAP functions in many important entral nervous system (CNS) processes, including cell communication and the functioning of the blood brain barrier. Improper GFAP regulation can cause multiple disorders. Defects in GFAP is related to Alexander disease which is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes.
References
Buniatian G, et al., 1998, Biology of the cell. 90(1): 53-61.Chen YS, et al., 2011, Experimental Cell Research. 317(16): 2252-66.Isaacs A, et al., 1998, Genomics. 51(1): 152-4.
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