Rat GDNF Protein (His Tag) (Cytokine)

Glial cell derived neurotrophic factor Protein Datasheet

GDNF Protein Product Information

• Synonym: GDNF
• Protein Construction: A DNA sequence encoding the mature form of rat GDNF (Q07731-1) (Ser78-Ile211) was expressed ,with a polyhistide tag at the N-terminus.
• Source: Rat
• Expression Host: Baculovirus-Insect cells

GDNF Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant rat GDNF consists of 150 amino acids and predicts a molecular mass of 17.1 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GDNF protein

GDNF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GDNF Protein Description

Glial cell derived neurotrophic factor (GDNF) belongs to the GDNF family of ligands(GFL). It is a founding member of GFL. GFL comprises four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN) and plays a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. GDNF is a highly conserved neurotrophic factor. It plays a key role in the promotion of the survival of dopaminergic neurons. The recombinant form of this protein promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. GDNF has been taken as a potent survival factor for central motoneurons and it can cause two Parkinson's disease clinical trial and a number of animal trials. GDNF may be a new target for drug addiction and alcoholism treatment.

References

1. Oppenheim RW. et al., 1995, Nature. 373 (6512): 344-6.
2. Tomac A. et al., 1995, Nature. 373 (6512): 335-9.
3. Schindelhauer D. et al., 1996, Genomics. 28 (3): 605-7.
4. Carnicella S. et al., 2008, Proc Natl Acad Sci. 105 (23): 8114-9.