Cynomolgus monkey GDF15 Gene cDNA Clone / ORF Clone

GDF15 cDNA Clone Datasheet

GDF15 cDNA Clone Product Information

• cDNA Size: 927
• Gene Synonym: GDF15
• Source: Cynomolgus monkey

GDF15 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) growth differentiation factor 15 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 88 G/A，350 A/C，353 G/C，494 A/C，resulting in the amino acid Val substitution by Met, Asp substitution by Ala, Cys substitution by Ser, Gln substitution by Pro and 231 C/T not causing the amino acid variation.
  Please check the sequence information before order.

GDF15 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GDF15 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GDF15 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCGGGGC AAGAACTCAA GACACTGAAT GGCTCTCAGA TGCTCTTGGT GTTGCTTGTG
61       CTCTTATGGC CGCCGCATGG GGGCGCCATG TCTCTGGCCG AGGCGAGCCG CGCAAGTTTC
121      CCGGGACCCT CAGACTTGCA CTCCGAAGAC TCCAGATTCC GAGAGTTGCG GAAACGCTAC
181      GAGGACCTGC TGACCAGGCT GCGGGCCAAC CAGAGCTGGG AAGATTCAAA TACCGACCTC
241      ATCCAGGCCC CTGAGGTCCG GATACTCACA CCAGAAGTGC GGCTGGGATC TGGCGGCCAC
301      CTGCACCTGC GTATCTCCCG GGCCGTCCTG CCCGAGGGGC TCCCCGAGGC CTCCCGCATT
361      CACCGGGCTC TGTTCCGGCT GTCCCCGACG GCGTCAAGGT CGTGGGACGT GACACGACCG
421      CTGCGGCGTC AGCTCAGGCT TGCAAGACCC CAGGCGCCCG CGCTGCACCT GCGACTGTCG
481      CCGCCGCCGT CGCCGTCGGA CCAACTGCTG GTAAAGTCTT CGTCCTCACG GCCCCAGCTG
541      GAGTTGCACT TGCGGCCGCG AGCCGCCAGG GGGCGCCGCA GAGCGCGTGC GCGCAACGGG
601      GACCGCTGTC CGCTCGGGCC CGGGCGCTGC TGCCGTCTGC ACACTGTCCA CGCGTCGCTG
661      GAAGACCTGG GCTGGGCCGA TTGGGTGCTG TCGCCACGGG AGGTGCAAGT GACCATGTGC
721      ATCGGCGCGT GCCCGAGCCA GTTCCGGGAG GCCAACATGC ACGCGCAGAT CAAGATGAAC
781      CTGCACCGCC TGAAGCCCGA CACGGTGCCA GCGCCCTGCT GCGTGCCCGC CAGCTACAAT
841      CCCATGGTGC TCATTCAAAA GACCGACACC GGGGTGTCAC TCCAGACCTA TGACGACTTG
901      TTAGCCAAAG ACTGCCACTG CGTGTGA

 

Quality Control:cDNA sequencing results is available upon request.