Human GDF-3 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10492-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM.020634.1

cDNA Size:   1095

Synonym:  GDF3

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens growth differentiation factor 3 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 637 G/A resulting in the amino acid 213Gly substitution by Arg.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCTTCGTT TCTTGCCAGA TTTGGCTTTC AGCTTCCTGT TAATTCTGGC TTTGGGCCAG
61       GCAGTCCAAT TTCAAGAATA TGTCTTTCTC CAATTTCTGG GCTTAGATAA GGCGCCTTCA
121      CCCCAGAAGT TCCAACCTGT GCCTTATATC TTGAAGAAAA TTTTCCAGGA TCGCGAGGCA
181      GCAGCGACCA CTGGGGTCTC CCGAGACTTA TGCTACGTAA AGGAGCTGGG CGTCCGCGGG
241      AATGTACTTC GCTTTCTCCC AGACCAAGGT TTCTTTCTTT ACCCAAAGAA AATTTCCCAA
301      GCTTCCTCCT GCCTGCAGAA GCTCCTCTAC TTTAACCTGT CTGCCATCAA AGAAAGGGAA
361      CAGTTGACAT TGGCCCAGCT GGGCCTGGAC TTGGGGCCCA ATTCTTACTA TAACCTGGGA
421      CCAGAGCTGG AACTGGCTCT GTTCCTGGTT CAGGAGCCTC ATGTGTGGGG CCAGACCACC
481      CCTAAGCCAG GTAAAATGTT TGTGTTGCGG TCAGTCCCAT GGCCACAAGG TGCTGTTCAC
541      TTCAACCTGC TGGATGTAGC TAAGGATTGG AATGACAACC CCCGGAAAAA TTTCGGGTTA
601      TTCCTGGAGA TACTGGTCAA AGAAGATAGA GACTCAAGGG TGAATTTTCA GCCTGAAGAC
661      ACCTGTGCCA GACTAAGATG CTCCCTTCAT GCTTCCCTGC TGGTGGTGAC TCTCAACCCT
721      GATCAGTGCC ACCCTTCTCG GAAAAGGAGA GCAGCCATCC CTGTCCCCAA GCTTTCTTGT
781      AAGAACCTCT GCCACCGTCA CCAGCTATTC ATTAACTTCC GGGACCTGGG TTGGCACAAG
841      TGGATCATTG CCCCCAAGGG GTTCATGGCA AATTACTGCC ATGGAGAGTG TCCCTTCTCA
901      CTGACCATCT CTCTCAACAG CTCCAATTAT GCTTTCATGC AAGCCCTGAT GCATGCCGTT
961      GACCCAGAGA TCCCCCAGGC TGTGTGTATC CCCACCAAGC TGTCTCCCAT TTCCATGCTC
1021     TACCAGGACA ATAATGACAA TGTCATTCTA CGACATTATG AAGACATGGT AGTCGATGAA
1081     TGTGGGTGTG GGTAG

Quality Control: cDNA sequencing results is available upon request.

Cytokine

TGF-beta