Human GCG Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13607-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

GCG cDNA Clone Datasheet

GCG cDNA Clone Product Information

Gene Bank Ref.ID:	BC005278
cDNA Size:	543
Gene Synonym:	GLP1;GLP2;GRPP;GCG
Source:	Human

GCG cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens glucagon DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

GCG cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GCG cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GCG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAAAGCA TTTACTTTGT GGCTGGATTA TTTGTAATGC TGGTACAAGG CAGCTGGCAA
61       CGTTCCCTTC AAGACACAGA GGAGAAATCC AGATCATTCT CAGCTTCCCA GGCAGACCCA
121      CTCAGTGATC CTGATCAGAT GAACGAGGAC AAGCGCCATT CACAGGGCAC ATTCACCAGT
181      GACTACAGCA AGTATCTGGA CTCCAGGCGT GCCCAAGATT TTGTGCAGTG GTTGATGAAT
241      ACCAAGAGGA ACAGGAATAA CATTGCCAAA CGTCACGATG AATTTGAGAG ACATGCTGAA
301      GGGACCTTTA CCAGTGATGT AAGTTCTTAT TTGGAAGGCC AAGCTGCCAA GGAATTCATT
361      GCTTGGCTGG TGAAAGGCCG AGGAAGGCGA GATTTCCCAG AAGAGGTCGC CATTGTTGAA
421      GAACTTGGCC GCAGACATGC TGATGGTTCT TTCTCTGATG AGATGAACAC CATTCTTGAT
481      AATCTTGCCG CCAGGGACTT TATAAACTGG TTGATTCAGA CCAAAATCAC TGACAGGAAA
541      TAA

Quality Control:cDNA sequencing results is available upon request.