GCA / Grancalcin Protein (His Tag)

Grancalcin, EF-hand calcium binding protein Protein Datasheet

GCA / Grancalcin Protein Product Information

• Synonym: GCL
• Protein Construction: A DNA sequence encoding the human GCA (P28676?) (Met 1 –Ile 217) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E. Coli

GCA / Grancalcin Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human GCA consisting of 228 amino acids and has a calculated molecular mass of 25.5KDa. It migrates as an 30 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris, pH 8.0
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GCA / Grancalcin protein

GCA / Grancalcin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GCA / Grancalcin Protein Description

Grancalcin (GCA) is a EF-hand Ca(2+)-binding protein, which is particularly abundant in neutrophils and monocytes, with relatively small amounts in lymphocytes. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin protein displays a Ca(2+)-dependent translocation to the granules and plasma membrane of neutrophils, suggesting that it might play an effector role in the specialized functions of these cells. Grancalcin localization is dependent upon calcium and magnesium. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. Recently, L-plastin, a leukocyte-specific actin-bundling protein, was found to interact with grancalcin in a negative Ca(2+)-dependent manner. In addition, Grancalcin plays a key role in leukocyte-specific functions that are responsible for host defense.

References

1. Boyhan A. et al., 1992, J Biol Chem. 267(5): 2928-33.
2. Lollike K. et al., 1995, J Immunol Methods. 185(1): 1-8.
3. Lollike K. et al., 2001, J Biol Chem. 276(21): 17762-9.
4. Liu F. et al., 2004, Biosci Biotechnol Biochem. 68(4): 894-902.