GBP1 Protein (His Tag)

Guanylate binding protein 1, interferon-inducible Protein Datasheet

GBP1 Protein Product Information

• Synonym: GBP1
• Protein Construction: A DNA sequence encoding the human GBP1 (AAA35871.1) (Met 1- Cys 589) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

GBP1 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human GBP1 comprises 600 amino acids and has a predicted molecular mass of 69 kDa. rhGBP1 migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: GBP1 protein

GBP1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GBP1 Protein Description

Guanylate-binding proteins (GBPs) are the most abundant cellular proteins expressed in response to interferon-γ(IFN-γ), with seven highly homologous members in humans, termed HuGBP-1 to HuGBP-7. GBP-1 is the best characterized GBP. GBP-1 expression is induced by type1 and type2 interferons, including IFN-γ and also by interleukin-1β(IL-1β), IL-1α, and tumor necrosis factor-α(TNF-α). Interferonγ-induced GTPases are key to the protective immunity against microbial and viral pathogens. GBP-1 is secreted from cells. Secretion occurred in the absence of a leader peptide via a nonclassical, likely ABC transporter-dependent, pathway, was independent of GBP-1 GTPase activity and isoprenylation, and did not require additional interferon-γ-induced factors. GBP-1 was only secreted from endothelial cells. Clinically most important was the detection of significantly increased GBP-1 concentrations in the cerebrospinal fluid of patients with bacterial meningitis as compared to control patients.

References

1. Tripal P. et al., 2007, J Interferon Cytokine Res. 27 (1): 44-52.
2. Naschberger E. et al., 2006, Am J Pathol. 169 (3): 1088-99.