Human GATA4 Gene cDNA Clone / ORF Clone

GATA4 cDNA Clone Datasheet

GATA4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002052.3
• cDNA Size: 1332
• Gene Synonym: MGC126629, GATA4
• Source: Human

GATA4 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens GATA binding protein 4 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the 617-619 TAG insertion mutation.

GATA4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GATA4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GATA4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTATCAGA GCTTGGCCAT GGCCGCCAAC CACGGGCCGC CCCCCGGTGC CTACGAGGCG
61       GGCGGCCCCG GCGCCTTCAT GCACGGCGCG GGCGCCGCGT CCTCGCCAGT CTACGTGCCC
121      ACACCGCGGG TGCCCTCCTC CGTGCTGGGC CTGTCCTACC TCCAGGGCGG AGGCGCGGGC
181      TCTGCGTCCG GAGGCGCCTC GGGCGGCAGC TCCGGTGGGG CCGCGTCTGG TGCGGGGCCC
241      GGGACCCAGC AGGGCAGCCC GGGATGGAGC CAGGCGGGAG CCGACGGAGC CGCTTACACC
301      CCGCCGCCGG TGTCGCCGCG CTTCTCCTTC CCGGGGACCA CCGGGTCCCT GGCGGCCGCC
361      GCCGCCGCTG CCGCGGCCCG GGAAGCTGCG GCCTACAGCA GTGGCGGCGG AGCGGCGGGT
421      GCGGGCCTGG CGGGCCGCGA GCAGTACGGG CGCGCCGGCT TCGCGGGCTC CTACTCCAGC
481      CCCTACCCGG CTTACATGGC CGACGTGGGC GCGTCCTGGG CCGCAGCCGC CGCCGCCTCC
541      GCCGGCCCCT TCGACAGCCC GGTCCTGCAC AGCCTGCCCG GCCGGGCCAA CCCGGCCGCC
601      CGACACCCCA ATCTCGTAGA TATGTTTGAC GACTTCTCAG AAGGCAGAGA GTGTGTCAAC
661      TGTGGGGCTA TGTCCACCCC GCTCTGGAGG CGAGATGGGA CGGGTCACTA TCTGTGCAAC
721      GCCTGCGGCC TCTACCACAA GATGAACGGC ATCAACCGGC CGCTCATCAA GCCTCAGCGC
781      CGGCTGTCCG CCTCCCGCCG AGTGGGCCTC TCCTGTGCCA ACTGCCAGAC CACCACCACC
841      ACGCTGTGGC GCCGCAATGC GGAGGGCGAG CCTGTGTGCA ATGCCTGCGG CCTCTACATG
901      AAGCTCCACG GGGTCCCCAG GCCTCTTGCA ATGCGGAAAG AGGGGATCCA AACCAGAAAA
961      CGGAAGCCCA AGAACCTGAA TAAATCTAAG ACACCAGCAG CTCCTTCAGG CAGTGAGAGC
1021     CTTCCTCCCG CCAGCGGTGC TTCCAGCAAC TCCAGCAACG CCACCACCAG CAGCAGCGAG
1081     GAGATGCGTC CCATCAAGAC GGAGCCTGGC CTGTCATCTC ACTACGGGCA CAGCAGCTCC
1141     GTGTCCCAGA CGTTCTCAGT CAGTGCGATG TCTGGCCATG GGCCCTCCAT CCACCCTGTC
1201     CTCTCGGCCC TGAAGCTCTC CCCACAAGGC TATGCGTCTC CCGTCAGCCA GTCTCCACAG
1261     ACCAGCTCCA AGCAGGACTC TTGGAACAGC CTGGTCTTGG CCGACAGTCA CGGGGACATA
1321     ATCACTGCGT AA

 

Quality Control:cDNA sequencing results is available upon request.