|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Homo sapiens glyceraldehyde-3-phosphate dehydrogenase DNA.|
|GAPDH, G3PD, GAPD, MGC88685|
|pMD18-T Simple Vector|
|Identical with the Gene Bank Ref. ID sequence except for two point mutations: 381 T/C and 885 T/C, none of which causes the encoded amino acid variation yet.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human GAPDH Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG10094-M-F|
|Human GAPDH Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG10094-M-H|
|Human GAPDH Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG10094-M-M|
|Human GAPDH Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG10094-M-N|
|Human GAPDH Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG10094-M-Y|
|Product name||Product name|
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.