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Human GAPDH Gene cDNA Clone (full-length ORF Clone)
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Human GAPDH Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG10094-M



  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Antibody Production Services

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_002046.3

cDNA Size:   1008

Synonym:   GAPDH, G3PD, GAPD, MGC88685

Source:  Human.

cDNA Description:   ORF Clone of Homo sapiens glyceraldehyde-3-phosphate dehydrogenase DNA

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG


Sequence Description:   Identical with the Gene Bank Ref. ID sequence except for two point mutations: 381 T/C and 885 T/C, none of which causes the encoded amino acid variation yet.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15–30 min .
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG
61       GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
121      AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
181      AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
241      GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
301      GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
361      ATCTCTGCCC CCTCTGCTGA CGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
421      GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
481      GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
541      ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
601      GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
661      ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
721      TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
781      GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
841      GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTCGACGC TGGGGCTGGC
901      ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
961      AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA


Quality Control:   cDNA sequencing results is available upon request.

 

GAPDH related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"