Human GAPDH Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10094-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_002046.3

cDNA Size:   1008

Synonym:   GAPDH, G3PD, GAPD, MGC88685

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens glyceraldehyde-3-phosphate dehydrogenase DNA

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 381 T/C and 885 T/C, none of which causes the encoded amino acid variation yet.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGGGAAGG TGAAGGTCGG AGTCAACGGA TTTGGTCGTA TTGGGCGCCT GGTCACCAGG
61       GCTGCTTTTA ACTCTGGTAA AGTGGATATT GTTGCCATCA ATGACCCCTT CATTGACCTC
121      AACTACATGG TTTACATGTT CCAATATGAT TCCACCCATG GCAAATTCCA TGGCACCGTC
181      AAGGCTGAGA ACGGGAAGCT TGTCATCAAT GGAAATCCCA TCACCATCTT CCAGGAGCGA
241      GATCCCTCCA AAATCAAGTG GGGCGATGCT GGCGCTGAGT ACGTCGTGGA GTCCACTGGC
301      GTCTTCACCA CCATGGAGAA GGCTGGGGCT CATTTGCAGG GGGGAGCCAA AAGGGTCATC
361      ATCTCTGCCC CCTCTGCTGA CGCCCCCATG TTCGTCATGG GTGTGAACCA TGAGAAGTAT
421      GACAACAGCC TCAAGATCAT CAGCAATGCC TCCTGCACCA CCAACTGCTT AGCACCCCTG
481      GCCAAGGTCA TCCATGACAA CTTTGGTATC GTGGAAGGAC TCATGACCAC AGTCCATGCC
541      ATCACTGCCA CCCAGAAGAC TGTGGATGGC CCCTCCGGGA AACTGTGGCG TGATGGCCGC
601      GGGGCTCTCC AGAACATCAT CCCTGCCTCT ACTGGCGCTG CCAAGGCTGT GGGCAAGGTC
661      ATCCCTGAGC TGAACGGGAA GCTCACTGGC ATGGCCTTCC GTGTCCCCAC TGCCAACGTG
721      TCAGTGGTGG ACCTGACCTG CCGTCTAGAA AAACCTGCCA AATATGATGA CATCAAGAAG
781      GTGGTGAAGC AGGCGTCGGA GGGCCCCCTC AAGGGCATCC TGGGCTACAC TGAGCACCAG
841      GTGGTCTCCT CTGACTTCAA CAGCGACACC CACTCCTCCA CCTTCGACGC TGGGGCTGGC
901      ATTGCCCTCA ACGACCACTT TGTCAAGCTC ATTTCCTGGT ATGACAACGA ATTTGGCTAC
961      AGCAACAGGG TGGTGGACCT CATGGCCCAC ATGGCCTCCA AGGAGTAA

Quality Control: cDNA sequencing results is available upon request.

Product Catalog

Human GAPDH Gene cDNA Clone / ORF Clone

GAPDH related areas, pathways, and other information