Anti-GAPDH Antibody

Cat: 100242-MM05
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Anti-GAPDH Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-GAPDH Antibody
Validated applications
Species reactivity
Reacts with: Human
A synthetic peptide corresponding to the center region of the human GAPDH
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the human GAPDH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #05
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GAPDH Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GAPDH Antibody (Mouse Monoclonal antibody) Images
Human GAPDH Western blot (WB) 6437

Anti-GAPDH mouse monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lane C: MCF7 Whole Cell Lysate

Lane D: MCF7 whole cell lysate

Lane E: PC-3 whole cell lysate

Lane F: Daudi whole cell lysate

Lane G: A549 whole cell lysate

Lane H: Rat heart tissue lysate

Lane I: Rat liver tissue lysate

Lane J: Rat spleen tissue lysate

Lane K: Rat lung tissue lysate

Lane L: Rat kidney tissue lysate

Lane M: Rat brain tissue lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size: kDa

Observed band size:38 kDa

Anti-GAPDH Antibody Alternative Names
Anti-G3PD Antibody;Anti-GAPD Antibody;Anti-HEL-S-162eP Antibody
GAPDH Background Information

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Full Name
glyceraldehyde-3-phosphate dehydrogenase
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-GAPDH Antibody Rabbit Monoclonal IHC-P 100242-R005
    Anti-GAPDH Antibody Rabbit Polyclonal WB,ELISA,IP 10094-T52
    Anti-GAPDH Antibody Rabbit Polyclonal IHC-P 100242-T08
    Anti-GAPDH Antibody Rabbit Polyclonal WB,IHC-P 100242-T40
    Anti-GAPDH Antibody Rabbit Polyclonal ELISA 10094-T16
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