**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-GAPDH mouse monoclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell Lysate
Lane B: Hela Whole Cell Lysate
Lane C: MCF7 Whole Cell Lysate
Lane D: MCF7 whole cell lysate
Lane E: PC-3 whole cell lysate
Lane F: Daudi whole cell lysate
Lane G: A549 whole cell lysate
Lane H: Rat heart tissue lysate
Lane I: Rat liver tissue lysate
Lane J: Rat spleen tissue lysate
Lane K: Rat lung tissue lysate
Lane L: Rat kidney tissue lysate
Lane M: Rat brain tissue lysate
Lysates/proteins at 30 μg per lane.
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size: kDa
Observed band size:38 kDa
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
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