Anti-GAPDH Antibody

Cat: 100242-T40
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Anti-GAPDH Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-GAPDH Antibody
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human GAPDH
Immunogen
A synthetic peptide corresponding to the center region of the human GAPDH
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the human GAPDH, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GAPDH Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-GAPDH Antibody (Rabbit Polyclonal antibody) Images
Human GAPDH Immunohistochemistry(IHC) 4163
Immunochemical staining of human GAPDH in human renal carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human GAPDH Immunohistochemistry(IHC) 4166
Immunochemical staining of human GAPDH in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human GAPDH Western blot (WB) 6438

Anti-GAPDH rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lane C: Mouse brain Tissue Lysate

Lane D: Rat brain Tissue Lysate

Lane E: Rabbit brain Tissue Lysate

Lane F: Rabbit spleen Tissue Lysate

Lane G: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:37 kDa

(We are unsure as to the identity of these extra bands.)

Anti-GAPDH Antibody Alternative Names
Anti-G3PD Antibody;Anti-GAPD Antibody;Anti-HEL-S-162eP Antibody
GAPDH Background Information

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Full Name
glyceraldehyde-3-phosphate dehydrogenase
References
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-GAPDH Antibody Mouse Monoclonal WB 100242-MM05
    Anti-GAPDH Antibody Rabbit Monoclonal IHC-P 100242-R005
    Anti-GAPDH Antibody Rabbit Polyclonal WB,ELISA,IP 10094-T52
    Anti-GAPDH Antibody Rabbit Polyclonal IHC-P 100242-T08
    Anti-GAPDH Antibody Rabbit Polyclonal ELISA 10094-T16
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