**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-GAPDH rabbit polyclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell Lysate
Lane B: MCF7 Whole Cell Lysate
Lane C: Mouse brain Tissue Lysate
Lane D: Rat brain Tissue Lysate
Lane E: Rabbit brain Tissue Lysate
Lane F: Rabbit spleen Tissue Lysate
Lane G: K562 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:36 kDa
Observed band size:37 kDa
(We are unsure as to the identity of these extra bands.)
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
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