Human GALC Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13681-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

GALC cDNA Clone Datasheet

GALC cDNA Clone Product Information

Gene Bank Ref.ID:	NM_000153.3
cDNA Size:	2058
Gene Synonym:	GALC
Source:	Human

GALC cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens galactosylceramidase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1921 A/G, resulting in the amino acid Thr substitution by Ala and 1698 A/T not causing the amino acid variation.

GALC cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

GALC cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

GALC cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGAGT GGCTACTCTC GGCTTCCTGG CAACGCCGAG CGAAAGCTAT GACTGCGGCC
61       GCGGGTTCGG CGGGCCGCGC CGCGGTGCCC TTGCTGCTGT GTGCGCTGCT GGCGCCCGGC
121      GGCGCGTACG TGCTCGACGA CTCCGACGGG CTGGGCCGGG AGTTCGACGG CATCGGCGCG
181      GTCAGCGGCG GCGGGGCAAC CTCCCGACTT CTAGTAAATT ACCCAGAGCC CTATCGTTCT
241      CAGATATTGG ATTATCTCTT TAAGCCGAAT TTTGGTGCCT CTTTGCATAT TTTAAAAGTG
301      GAAATAGGTG GTGATGGGCA GACAACAGAC GGCACTGAGC CCTCCCACAT GCATTATGCA
361      CTAGATGAGA ATTATTTCCG AGGATACGAG TGGTGGTTGA TGAAAGAAGC TAAGAAGAGG
421      AATCCCAATA TTACACTCAT TGGGTTGCCA TGGTCATTCC CTGGATGGCT GGGAAAAGGT
481      TTCGACTGGC CTTATGTCAA TCTTCAGCTG ACTGCCTATT ATGTCGTGAC CTGGATTGTG
541      GGCGCCAAGC GTTACCATGA TTTGGACATT GATTATATTG GAATTTGGAA TGAGAGGTCA
601      TATAATGCCA ATTATATTAA GATATTAAGA AAAATGCTGA ATTATCAAGG TCTCCAGCGA
661      GTGAAAATCA TAGCAAGTGA TAATCTCTGG GAGTCCATCT CTGCATCCAT GCTCCTTGAT
721      GCCGAACTCT TCAAGGTGGT TGATGTTATA GGGGCTCATT ATCCTGGAAC CCATTCAGCA
781      AAAGATGCAA AGTTGACTGG GAAGAAGCTT TGGTCTTCTG AAGACTTTAG CACTTTAAAT
841      AGTGACATGG GTGCAGGCTG CTGGGGTCGC ATTTTAAATC AGAATTATAT CAATGGCTAT
901      ATGACTTCCA CAATCGCATG GAATTTAGTG GCTAGTTACT ATGAACAGTT GCCTTATGGG
961      AGATGCGGGT TGATGACGGC CCAGGAGCCA TGGAGTGGGC ACTACGTGGT AGAATCTCCT
1021     GTCTGGGTAT CAGCTCATAC CACTCAGTTT ACTCAACCTG GCTGGTATTA CCTGAAGACA
1081     GTTGGCCATT TAGAGAAAGG AGGAAGCTAC GTAGCTCTGA CTGATGGCTT AGGGAACCTC
1141     ACCATCATCA TTGAAACCAT GAGTCATAAA CATTCTAAGT GCATACGGCC ATTTCTTCCT
1201     TATTTCAATG TGTCACAACA ATTTGCCACC TTTGTTCTTA AGGGATCTTT TAGTGAAATA
1261     CCAGAGCTAC AGGTATGGTA TACCAAACTT GGAAAAACAT CCGAAAGATT TCTTTTTAAG
1321     CAGCTGGATT CTCTATGGCT CCTTGACAGC GATGGCAGTT TCACACTGAG CCTGCATGAA
1381     GATGAGCTGT TCACACTCAC CACTCTCACC ACTGGTCGCA AAGGCAGCTA CCCGCTTCCT
1441     CCAAAATCCC AGCCCTTCCC AAGTACCTAT AAGGATGATT TCAATGTTGA TTACCCATTT
1501     TTTAGTGAAG CTCCAAACTT TGCTGATCAA ACTGGTGTAT TTGAATATTT TACAAATATT
1561     GAAGACCCTG GCGAGCATCA CTTCACGCTA CGCCAAGTTC TCAACCAGAG ACCCATTACG
1621     TGGGCTGCCG ATGCATCCAA CACAATCAGT ATTATAGGAG ACTACAACTG GACCAATCTG
1681     ACTATAAAGT GTGATGTTTA CATAGAGACC CCTGACACAG GAGGTGTGTT CATTGCAGGA
1741     AGAGTAAATA AAGGTGGTAT TTTGATTAGA AGTGCCAGAG GAATTTTCTT CTGGATTTTT
1801     GCAAATGGAT CTTACAGGGT TACAGGTGAT TTAGCTGGAT GGATTATATA TGCTTTAGGA
1861     CGTGTTGAAG TTACAGCAAA AAAATGGTAT ACACTCACGT TAACTATTAA GGGTCATTTC
1921     GCCTCTGGCA TGCTGAATGA CAAGTCTCTG TGGACAGACA TCCCTGTGAA TTTTCCAAAG
1981     AATGGCTGGG CTGCAATTGG AACTCACTCC TTTGAATTTG CACAGTTTGA CAACTTTCTT
2041     GTGGAAGCCA CACGCTAA

Quality Control:cDNA sequencing results is available upon request.