Anti-GCSF Receptor Antibody (Mouse Monoclonal antibody) General Information
Anti-GCSF Receptor Antibody
Reacts with: Human
Human GCSF Receptor
Human cell-derived rhG-CSFR extracellular domain (Catalog#10218-HCCH)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human G-CSFR extracellular domain (rhG-CSFR; Catalog #10218-HCCH; aa 1-621; NP_000751.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #2D9G1E3
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-GCSF Receptor Antibody (Mouse Monoclonal antibody) Validated Applications
||This antibody will detect Human G-CSF R / CD114 / CSF3R in ELISA pair set (Catalog: # SEK10218). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10218-MM06).
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-GCSF Receptor Antibody Alternative Names
Anti-CD114 Antibody;Anti-CSF3R Antibody;Anti-G-CSF R Antibody;Anti-GCSFR Antibody
GCSF Receptor Background Information
Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis.
colony stimulating factor 3 receptor (granulocyte)
Kasper B, et al. (1999) Association of src-kinase Lyn and non-src-kinase Syk with the granulocyte colony-stimulating factor receptor (G-CSFR) is not abrogated in neutrophils from severe congenital neutropenia patients with point mutations in the G-CSFR mRNA. Int J Hematol. 70(4): 241-7.Hollenstein U, et al. (2000) Endotoxin down-modulates granulocyte colony-stimulating factor receptor (CD114) on human neutrophils. J Infect Dis. 182(1): 343-6.Kindwall-Keller TL, et al. (2008) Role of the proteasome in modulating native G-CSFR expression. Cytokine. 43(2): 114-23.Beel K, et al. (2009) G-CSF receptor (CSF3R) mutations in X-linked neutropenia evolving to acute myeloid leukemia or myelodysplasia. Haematologica. 94(10): 1449-52.