Mouse CX3CL1 Gene cDNA Clone / ORF Clone

CX3CL1 cDNA Clone Datasheet

CX3CL1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009142.3
• cDNA Size: 1188
• Gene Synonym: CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e; Cx3cl1
• Source: Mouse

CX3CL1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus chemokine (C-X3-C motif) ligand 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CX3CL1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CX3CL1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CX3CL1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCCCT CGCCGCTCGC GTGGCTGCTG CGCCTGGCCG CGTTCTTCCA TTTGTGTACT
61       CTGCTGCCGG GTCAGCACCT CGGCATGACG AAATGCGAAA TCATGTGCGA CAAGATGACC
121      TCACGAATCC CAGTGGCTTT GCTCATCCGC TATCAGCTAA ACCAGGAGTC CTGCGGCAAG
181      CGTGCCATTG TCCTGGAGAC GACACAGCAC AGACGCTTCT GTGCTGACCC GAAGGAGAAA
241      TGGGTCCAAG ACGCCATGAA GCATCTGGAT CACCAGGCTG CTGCCCTCAC TAAAAATGGT
301      GGCAAGTTTG AGAAGCGGGT GGACAATGTG ACACCTGGGA TCACCTTGGC CACTAGGGGA
361      CTGTCCCCAT CTGCCCTGAC AAAGCCTGAA TCCGCCACAT TGGAAGACCT TGCTTTGGAA
421      CTGACTACTA TTTCCCAGGA GGCCAGGGGG ACCATGGGGA CTTCCCAAGA GCCACCGGCA
481      GCAGTGACCG GATCATCTCT CTCAACTTCC GAGGCACAGG ATGCAGGGCT TACGGCTAAG
541      CCTCAGAGCA TTGGAAGTTT TGAGGCGGCT GACATCTCCA CCACCGTTTG GCCGAGTCCT
601      GCTGTCTACC AATCTGGATC TAGCTCCTGG GCTGAGGAAA AAGCTACTGA GTCCCCCTCC
661      ACTACAGCCC CATCTCCTCA GGTGTCCACT ACTTCACCTT CAACCCCAGA GGAAAATGTT
721      GGGTCCGAAG GCCAACCCCC ATGGGTCCAG GGACAGGACC TCAGTCCAGA GAAGTCTCTA
781      GGGTCTGAGG AGATAAACCC AGTTCATACT GATAATTTCC AGGAGAGGGG GCCTGGCAAC
841      ACAGTCCACC CCTCAGTGGC TCCCATCTCC TCTGAAGAGA CCCCCAGCCC AGAGCTGGTG
901      GCCTCGGGCA GCCAGGCTCC TAAGATAGAG GAACCCATCC ATGCCACTGC AGATCCCCAG
961      AAACTGAGTG TGCTTATCAC TCCTGTCCCC GACACCCAGG CAGCCACAAG GAGGCAGGCA
1021     GTGGGGCTAC TGGCTTTCCT TGGTCTTCTT TTCTGCCTAG GGGTGGCCAT GTTTGCTTAC
1081     CAGAGCCTTC AGGGCTGTCC CCGCAAAATG GCGGGGGAAA TGGTAGAAGG CCTCCGCTAC
1141     GTCCCCCGTA GCTGTGGCAG TAACTCATAC GTCCTGGTGC CAGTGTGA

 

Quality Control:cDNA sequencing results is available upon request.