Fractalkine / CX3CL1 Antibody (Cytokine)

Fractalkine / CX3CL1 Antibody Datasheet

Fractalkine / CX3CL1 Antibody Product Information

• Immunogen: Recombinant Human Fractalkine / CX3CL1 protein (Catalog#10636-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 12
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Fractalkine / CX3CL1 (rh Fractalkine / CX3CL1; Catalog#10636-H08H; NP_002987.1; Met 1-Arg 339).

Fractalkine / CX3CL1 Antibody Usage Guide

• Specificity: Human Fractalkine / CX3CL1
• Flow Cytometry:
  

  Flow cytometric analysis of Human CX3CL1 expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-CX3CL1 (10636-MM12, 1 μg/test), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Fractalkine / CX3CL1 Antibody Background

Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein containing multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known as fractalkine (in humans) and neurotactin (in mice). The polypeptide structure of CXC3L1 differs from the typical structure of other chemokines. Mature human CX3CL1 consists of a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound CX3CL1 promotes strong adhesion of leukocytes to activated endothelial cells where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1.

References

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3. Nomiyama, H. et al., 1998, Cytogenet. Cell Genet. 81: 10-11.
4. Papadopoulos, E. et al., 1999, Eur. J. Immunol. 29 (8): 2551-2559.
5. Umehara, H. et al., 2004, Arterioscler. Thromb. Vasc. Biol. 24 (1): 34-40.