Anti-Fractalkine Antibody (Mouse Monoclonal antibody) General Information
Reacts with: Human
Recombinant Human Fractalkine / CX3CL1 protein (Catalog#10636-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Fractalkine / CX3CL1 (rh Fractalkine / CX3CL1; Catalog#10636-H08H; NP_002987.1; Met 1-Arg 339).
Monoclonal Mouse IgG2a Clone #12
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Fractalkine Antibody (Mouse Monoclonal antibody) Validated Applications
|| 0.5-2 μg/Test
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-Fractalkine Antibody (Mouse Monoclonal antibody) Images
Flow cytometric analysis of Human CX3CL1 expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-CX3CL1 (10636-MM12), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-Fractalkine Antibody Alternative Names
Anti-ABCD-3 Antibody;Anti-C3Xkine Antibody;Anti-CXC3 Antibody;Anti-CXC3C Antibody;Anti-fractalkine Antibody;Anti-neurotactin Antibody;Anti-NTN Antibody;Anti-NTT Antibody;Anti-SCYD1 Antibody
Fractalkine Background Information
Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.
chemokine (C-X3-C motif) ligand 1
Imai T, et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 91(4): 521-30.Papadopoulos EJ, et al. (1999) Fractalkine, a CX3C chemokine, is expressed by dendritic cells and is up-regulated upon dendritic cell maturation. Eur J Immunol. 29 (8): 2551-9.Umehara H, et al. (2004) Fractalkine in vascular biology: from basic research to clinical disease". Arterioscler. Thromb Vasc Biol. 24 (1): 34-40.