Fn14 / TNFRSF12A Protein (Cytokine)

Tumor necrosis factor receptor superfamily, member 12A Protein Datasheet

Fn14 / TNFRSF12A Protein Product Information

• Synonym: CD266, FN14, TWEAKR
• Protein Construction: A DNA sequence encoding the human TNFRSF12A isoform 1 (Q9NP84-1) extracellular domain (Glu 28-Trp 79) was fused with the Fc region of human IgG1 at the N-terminus.
• Source: Human
• Expression Host: Human Cells

Fn14 / TNFRSF12A Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu
• Molecular Mass: The recombinant human TNFRSF12A/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 313 amino acids and has a calculated molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTNFRSF12A/Fc monomer is approximately 37 kDa.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Fn14 / TNFRSF12A protein

Fn14 / TNFRSF12A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Fn14 / TNFRSF12A Protein Description

Fn14 (tumor necrosis factor receptor superfamily, member 12A), also known as TNFRSF12A, is the receptor for TNFSF12/TWEAK. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis. The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. Fn14 can be detected in heart, placenta, kidney, lung, skeletal muscle, pancreas, brain and liver. It is the weak inducer of apoptosis in some cell types. Fn14 promotes angiogenesis and the proliferation of endothelial cells. It may modulate cellular adhesion to matrix proteins.

References

1. Burkly LC. et al., 2011, Adv Exp Med Biol. 691:305-22.
2. Huang M. et al., 2011, Carcinogenesis. 32(11):1589-96.
3. Dharmapatni AA. et al., 2011, Arthritis Res Ther. 13(2):R51.