GFPSpark® & OFPSpark® tagged Expression ORF Clones

Sinobiological has constructed expression-ready plasmids which the target gene is tagged with GFPSpark®/ OFPSpark® in N- or C-terminal. Fluorescent proteins as genetically encoded tools are used extensively by life scientists. Almost all endogenous proteins of interest can be fused to fluorescent proteins. The fusion proteins can direct visualization of the organelles or structures by fluorescence microscopy in mammalian cells.
The main applications of fluorescent proteins include assay promoter activity,organelle marker,protein interaction,drug screening,drganism labeling,sensors.

Sino Biological Inc. recommend with organelle marker expression vector:

1) GFP or OFP fluorescent colors for choice
2) Easy labeling of cell compartments and proteins
3) Perfect for study of localization and trafficking
4) No antibody or chemical treatment required

Fluorescent Protein

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and gene was first cloned in 1992. The first demonstration of its utility as a fluorescent tag for in vivo labeling in 1994,and since then a keen interest in the structure, biochemistry, and biophysics of GFP-like fluorescent proteins, which resulted in an avalanche of scientific publications on FPs and their applications to solve basic problems in molecular and cell biology.Sinobio has self-developed Green fluorescent protein GFPSpark® and Red (orange) fluorescent protein OFPSpark®.

OFPSpark® is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark® is more than twice brighter than mOrange2. Fast OFPSpark® maturation makes it clearly detectable in mammalian cells as early as within 8hrs after transfection.
- Bright fluorescence
- High pH-stability
- Fast maturation
- Recommended for gene expression analysis, cell labeling and organelle labeling

Green Fluorescent Protein GFPSpark® Orange/Red Fluorescencent Protein OFPSpark®

GFPSpark® & OFPSpark® tag cDNA ORF

• Higher protein expression level: enhanced CMV promoter
• multiple tags for choice: C-,N-GFPSpark®/OFPSpark®
• full-length sequence confirmed
• mammalian expression, stable or transient
• expression validated
Here are the Expression cDNA ORF Maps:  

GFPSpark® & OFPSpark® tag cDNA ORF expression validated

GFPSpark® comparison with EGFP: Brighter fluorescence OFPSpark® comparison with commercial orange RFP: Brighter fluorescence
A) pCMV3-GFPSpark® and pCMV3-EGFP recombinant plasmids transiently expression in 293 H cell.The green fluorescent signals can be detected in cytoplasm under the excitation channel of 455~495 nm by Microscopy. GFPSpark® gives the brighter signal 24h after transfection.
B) The TUBB gene tagged with GFPSpark® and EGFP on pCMV3 vector
C) The TOMM20 gene tagged with GFPSpark® and EGFP on pCMV3 vector
These different expression vector transfected Hela cells transiently. After 48h, the strong green fluorescent signals can be detected by laser scanning confocal microscope(Nikon).The fluorecene intensity of GFPSpark® is better than EGFP.
OFPSpark®,commercial orange Red recombinant plasmids were constructed on pCMV3 vector.Transiently transfected 293H cells, the red and orange fluorescent signals can be detected in cytoplasm under the excitation channel of 532.5~587.5 nm, OFPSpark® gives the brighter signal 48h after transfection.

GFP/OFP-tagged Expression-ready cDNA ORF Clones for Organelle Marker

The recombinant plasmids containing different subcellular localization genes were constructed on pCMV3 vector.These different expression vector transfected Hela cells transiently. After 48h, the fluorescent signals can be detected by laser scanning confocal microscope(Nikon) (GFPSpark® excitation/ emission max = 487 / 508 nm, OFPSpark® excitation/emission maxima =549 / 566 nm).

HG11111-ANG HG11215-ACG HG12063-ACG HG11277-ACG
HG12282-ACR HG14013-ANG HG11626-ACR HG11263-ACR

GFPSpark® & OFPSpark® tag cDNA ORF list

Organelle Gene Catalog No. Description Fluorescent Protein Shopping
Autophagosome ATG12/apg12 HG11111-ACG GFPSpark fusion with ATG12 C terminal Green In Stock
ATG12/apg12 HG11111-ACR OFPSpark fusion with ATG12 C terminal Orange/Red In Stock
LC3A/MAP1LC3A HG14322-ACG GFPSpark fusion with MAP1LC3A C terminal Green In Stock
LC3A/MAP1LC3A HG14322-ACR OFPSpark fusion with MAP1LC3A C terminal Orange/Red In Stock
LC3B/MAP1LC3B HG14555-ACG GFPSpark fusion with MAP1LC3B C terminal Green In Stock
LC3B/MAP1LC3B HG14555-ACR OFPSpark fusion with MAP1LC3B C terminal Orange/Red In Stock
ARHI/DIRAS3 HG14239-ACG GFPSpark fusion with DIRAS3 C terminal Green In Stock
ARHI/DIRAS3 HG14239-ACR OFPSpark fusion with DIRAS3 C terminal Orange/Red In Stock

Fluorescent Protein Related Products

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• Real Time Quantitative PCR Analysis