Sinobiological has constructed expression-ready plasmids which the target gene is tagged with GFPSpark®/ OFPSpark® in N- or C-terminal. Fluorescent proteins as genetically encoded tools are used extensively by life scientists. Almost all endogenous proteins of interest can be fused to fluorescent proteins. The fusion proteins can direct visualization of the organelles or structures by fluorescence microscopy in mammalian cells.
The main applications of fluorescent proteins include assay promoter activity,organelle marker,protein interaction,drug screening,drganism labeling,sensors.
1) GFP or OFP fluorescent colors for choice
2) Easy labeling of cell compartments and proteins
3) Perfect for study of localization and trafficking
4) No antibody or chemical treatment required
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and gene was first cloned in 1992. The first demonstration of its utility as a fluorescent tag for in vivo labeling in 1994,and since then a keen interest in the structure, biochemistry, and biophysics of GFP-like fluorescent proteins, which resulted in an avalanche of scientific publications on FPs and their applications to solve basic problems in molecular and cell biology.Sinobio has self-developed Green fluorescent protein GFPSpark® and Red (orange) fluorescent protein OFPSpark®.
OFPSpark® is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark® is more than twice brighter than mOrange2. Fast OFPSpark® maturation makes it clearly detectable in mammalian cells as early as within 8hrs after transfection.
- Bright fluorescence
- High pH-stability
- Fast maturation
- Recommended for gene expression analysis, cell labeling and organelle labeling
|Green Fluorescent Protein GFPSpark®||Orange/Red Fluorescencent Protein OFPSpark®|
|• Higher protein expression level: enhanced CMV promoter
• multiple tags for choice: C-,N-GFPSpark®/OFPSpark®
• full-length sequence confirmed
|• mammalian expression, stable or transient
• expression validated
|Here are the Expression cDNA ORF Maps:|
|GFPSpark® comparison with EGFP: Brighter fluorescence||OFPSpark® comparison with commercial orange RFP: Brighter fluorescence|
|A) pCMV3-GFPSpark® and pCMV3-EGFP recombinant plasmids transiently expression in 293 H cell.The green fluorescent signals can be detected in cytoplasm under the excitation channel of 455~495 nm by Microscopy. GFPSpark® gives the brighter signal 24h after transfection.
B) The TUBB gene tagged with GFPSpark® and EGFP on pCMV3 vector
C) The TOMM20 gene tagged with GFPSpark® and EGFP on pCMV3 vector
These different expression vector transfected Hela cells transiently. After 48h, the strong green fluorescent signals can be detected by laser scanning confocal microscope(Nikon).The fluorecene intensity of GFPSpark® is better than EGFP.
|OFPSpark®,commercial orange Red recombinant plasmids were constructed on pCMV3 vector.Transiently transfected 293H cells, the red and orange fluorescent signals can be detected in cytoplasm under the excitation channel of 532.5~587.5 nm, OFPSpark® gives the brighter signal 48h after transfection.|
The recombinant plasmids containing different subcellular localization genes were constructed on pCMV3 vector.These different expression vector transfected Hela cells transiently. After 48h, the fluorescent signals can be detected by laser scanning confocal microscope(Nikon) (GFPSpark® excitation/ emission max = 487 / 508 nm, OFPSpark® excitation/emission maxima =549 / 566 nm).
|Organelle||Gene||Catalog No.||Description||Fluorescent Protein||Shopping|
|Autophagosome||ATG12/apg12||HG11111-ACG||GFPSpark fusion with ATG12 C terminal||Green||In Stock|
|ATG12/apg12||HG11111-ACR||OFPSpark fusion with ATG12 C terminal||Orange/Red||In Stock|
|LC3A/MAP1LC3A||HG14322-ACG||GFPSpark fusion with MAP1LC3A C terminal||Green||In Stock|
|LC3A/MAP1LC3A||HG14322-ACR||OFPSpark fusion with MAP1LC3A C terminal||Orange/Red||In Stock|
|LC3B/MAP1LC3B||HG14555-ACG||GFPSpark fusion with MAP1LC3B C terminal||Green||In Stock|
|LC3B/MAP1LC3B||HG14555-ACR||OFPSpark fusion with MAP1LC3B C terminal||Orange/Red||In Stock|
|ARHI/DIRAS3||HG14239-ACG||GFPSpark fusion with DIRAS3 C terminal||Green||In Stock|
|ARHI/DIRAS3||HG14239-ACR||OFPSpark fusion with DIRAS3 C terminal||Orange/Red||In Stock|