Fibronectin Fragment 2 Antibody

Human Fibronectin Fragment 2 / Fibronectin Antibody

Fibronectin Fragment 2 Antibody Product Information

• Immunogen: Recombinant human Fibronectin Fragment 2 protein ( Catalog#10314-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 014
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human Fibronectin Fragment 2 ( Catalog#10314-H08H ; Ser 607 - Pro 1265 ; CAD91166 ).

Fibronectin Fragment 2 Antibody Usage Guide

• Specificity: Human Fibronectin Fragment 2
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human Fibronectin Fragment 2 in WB. Using a DAB detection system, the detection limit for human Fibronectin Fragment 2 is approximately 0.5 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human Fibronectin Fragment 2. The detection limit for human Fibronectin Fragment 2 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Fibronectin Fragment 2 Antibody Background

Fibronectin ( FN ) is a large glycoprotein present as soluble disulfide-linked dimeric form in plasma and body fluids, and as dimeric or multimeric form at the cell surface or in extracellular matrix ( ECM ). Each monomer of FN consists of three types of homologous repeating units, that is 12 type I repeats, two type II repeats and 15-17 type III repeats. The occurrence of multiple isoforms results from alternative mRNA splicing of the ED-A, ED-B and III-CS regions, and subsequent post-translational modification. As an ECM component and one of the primary cell adhesion molecules, Fibronectin can be a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, as well as many integrin receptors through which FN mediates the variety of cellular signaling pathways. Accordingly, Fibronectin is involved in cell adhesion and migration, growth and differentiation, morphogenesis, wound healing, blood coagulation, host defense, and metastasis. Conversion of the non-functional soluble fibronectin to fibronectin fibrils requires conformational changes and exposure of cryptic epitopes necessary for polymerization. The fragment 2 of FN comprises the first 7 FN type III repeats and is suggested to be important for self association.

References

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2. Sechler, J.L. et al., 2001, J. Cell Biol. 154:1081-8.
3. Williams, C.M. et al., 2008, Cancer. Res. 68:3185-92.
4. Rounmen, P. et al., 2002, J. Cell. Sci. 115: 3861-3.