Anti-Fibronectin Antibody

Cat: 10314-RP03
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Anti-Fibronectin Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Fibronectin Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Fibronectin
Recombinant Human Fibronectin Fragment 2 protein (Catalog#10314-H08H)
Produced in rabbits immunized with purified, recombinant Human Fibronectin Fragment 2 (Catalog#10314-H08H; Ser 607-Pro 1265; CAD91166). Fibronectin Fragment 2 specific IgG was purified by human Fibronectin Fragment 2 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Fibronectin Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Fibronectin Antibody (Rabbit Polyclonal antibody) Images
Human Fibronectin Immunoprecipitation(IP) 7972

FF2 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-FF2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-FF2 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 262 kDa

Observed band size: 262 kDa

Human Fibronectin Western blot (WB) 13181

Anti-FF2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:233 kDa

Observed band size:230 kDa

Anti-Fibronectin Antibody Alternative Names
Anti-CIG Antibody;Anti-ED-B Antibody;Anti-FINC Antibody;Anti-FN Antibody;Anti-FNZ Antibody;Anti-GFND Antibody;Anti-GFND2 Antibody;Anti-LETS Antibody;Anti-MSF Antibody
Fibronectin Background Information

Fibronectin (FN) is a glycoprotein component of the extracellular matrix of the extracellular matrix (ECM) with roles in embryogenesis, development, and wound healing. More recently, FN has emerged as player in platelet thrombus formation and diseases associated with thrombosis including vascular remodeling, atherosclerosis, and cardiac repair following a myocardial infarct. Each monomer of FN consists of three types of homologous repeating units, that is 12 type I repeats, two type II repeats and 15-17 type III repeats. The occurrence of multiple isoforms results from alternative mRNA splicing of the ED-A, ED-B and III-CS regions, and subsequent post-translational modification. As an ECM component and one of the primary cell adhesion molecules, Fibronectin can be a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, as well as many integrin receptors through which FN mediates the variety of cellular signaling pathways. The study of solid human tumors showed among the early signs of malignant transformation the fragmentation of pericellular FN, concommitent with the increase of its production by the peritumoral stroma. These results should encourage further investigations concerning the potential importance of Fn production and breakdown during cancer progression. FN1 expression has been described to increase significantly from the morula towards the early blastocyst stage, suggesting that FN1 may also be involved in early blastocyst formation. The fragment 2 of FN comprises the first 7 FN type III repeats and is suggested to be important for self association during fibril growth via the key module III2.

Full Name
fibronectin 1
  • Labat-Robert J. (2002) Fibronectin in malignancy. Semin Cancer Biol. 12(3): 187-95.
  • Goossens K, et al. (2009) Quantification of fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos. BMC Dev Biol. 9: 1.
  • Maurer LM, et al. (2010) Emerging roles of fibronectin in thrombosis. Thromb Res. 125(4): 287-91.
  • Product Description Host Clonality Application Catalog# (PDF)
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    Anti-Fibronectin Antibody Rabbit Monoclonal WB,ELISA,IP 10314-R014
    Anti-Fibronectin Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 10314-T50
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